Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy

Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy Full article

Journal

Bioorganic Chemistry
ISSN: 0045-2068 , E-ISSN: 1090-2120

Output data

Year: 2019, Volume: 87, Pages: 876-887 Pages count : 12 DOI: 10.1016/j.bioorg.2018.11.052

Tags

Triterpene acids; Ursolic acid; Fluorescence; Cytotoxicity; Akt1/protein kinase B; MDM2/E3 ubiquitin-protein ligase

Authors

Frolova Tatiana S. ^1,2,3,4 , Lipeeva Alla, V ² , Baev Dmitry S. ^2,3 , Baiborodin Sergey, I ¹ , Orishchenko Konstantin E. ¹ , Kochetov Alexey, V ^1,3 , Sinitsyna Olga, I ^1,3

Affiliations

1	(Данные Web of science) Russian Acad Sci, Fed Res Ctr, Inst Cytol & Genet, Siberian Branch, 10 Lavrentyev Ave, Novosibirsk 630090, Russia
2	(Данные Web of science) Russian Acad Sci, Novosibirsk Inst Organ Chem, Siberian Branch, 10 Lavrentyev Ave, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, 2 Pirogov St, Novosibirsk 630090, Russia
4	(Данные Web of science) Russian Acad Sci, Fed Res Ctr Fundamental & Translat Med, Siberian Branch, 2 Timakov St, Novosibirsk 630117, Russia

Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series - ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B - a protein that inhibits apoptosis - is located.

Frolova T.S. , Lipeeva A.V. , Baev D.S. , Baiborodin S.I. , Orishchenko K.E. , Kochetov A.V. , Sinitsyna O.I.
Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy
Bioorganic Chemistry. 2019. V.87. P.876-887. DOI: 10.1016/j.bioorg.2018.11.052 WOS Scopus РИНЦ

Published print:

Jun 1, 2019

Web of science	WOS:000469026400085
Scopus	2-s2.0-85057730940
Elibrary	41790492
OpenAlex	W2902534637