Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy

Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy Full article

Journal

Journal of the American Chemical Society
ISSN: 0002-7863

Output data

Year: 2014, Volume: 136, Number: 28, Pages: 9874-9877 Pages count : DOI: 10.1021/ja505122n

Authors

Shevelev Georgiy Yu. ^1,4 , Krumkacheva Olesya A. ^2,4 , Lomzov Alexander A. ^1,4 , Kuzhelev Andrey A. ^3,4 , Rogozhnikova Olga Yu. ³ , Trukhin Dmitry V. ³ , Troitskaya Tatiana I. ³ , Tormyshev Victor M. ^3,4 , Fedin Matvey V. ^2,4 , Pyshnyi Dmitrii V. ^1,4 , Bagryanskaya Elena G. ^2,3,4

Affiliations

1	(Данные Web of science) SB RAS, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
2	(Данные Web of science) SB RAS, Int Tomog Ctr, Novosibirsk 630090, Russia
3	(Данные Web of science) SB RAS, NN Vorozhtsov Novosibirsk Inst Organ Chem, Novosibirsk 630090, Russia
4	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia

Resolving the nanometer-scale structure of biomolecules in natural conditions still remains a challenging task. We report the first distance measurement in nucleic acid at physiological temperature using electron paramagnetic resonance (EPR). The model 10-mer DNA duplex has been labeled with reactive forms of triarylmethyl radicals and then immobilized on a sorbent in water solution and investigated by double quantum coherence EPR. We succeeded in development of optimal triarylmethyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure that, working together, allowed us to measure as long distances as similar to 4.6 nm with high accuracy at 310 K (37 degrees C).

Shevelev G.Y. , Krumkacheva O.A. , Lomzov A.A. , Kuzhelev A.A. , Rogozhnikova O.Y. , Trukhin D.V. , Troitskaya T.I. , Tormyshev V.M. , Fedin M.V. , Pyshnyi D.V. , Bagryanskaya E.G.
Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy
Journal of the American Chemical Society. 2014. V.136. N28. P.9874-9877. DOI: 10.1021/ja505122n WOS Scopus

Published print:

Jul 16, 2014

Web of science	WOS:000339228200020
Scopus	2-s2.0-84904438581
OpenAlex	W2029758547