Chelidonic Acid and Its Derivatives from Saussurea Controversa: Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro

Chelidonic Acid and Its Derivatives from Saussurea Controversa: Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro Full article

Journal

Biomolecules
ISSN: 2218-273X

Output data

Year: 2019, Volume: 9, Number: 5, Article number : 189, Pages count : DOI: 10.3390/biom9050189

Tags

Saussurea controversa DC (Asteraceae); chelidonic acid; 4-oxo-4H-pyran-2; 6-dicarboxylic acid esters; X-ray diffraction analysis; human multipotent mesenchymal stromal cells

Authors

Avdeeva Elena ¹ , Shults Elvira ^2,3 , Rybalova Tatyana ^3,4 , Reshetov Yaroslav ¹ , Porokhova Ekaterina ⁵ , Sukhodolo Irina ⁵ , Litvinova Larisa ⁶ , Shupletsova Valeria ⁶ , Khaziakhmatova Olga ⁶ , Khlusov Igor ^5,7 , Guryev Artem ¹ , Belousov Mikhail ^1,7

Affiliations

1	(Данные Web of science) Siberian State Med Univ, Dept Pharmaceut Anal, Tomsk 634050, Russia
2	(Данные Web of science) Novosibirsk Inst Organ Chem, Lab Med Chem, Siberian Branch, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, 2 Pirogova St, Novosibirsk 630090, Russia
4	(Данные Web of science) Novosibirsk Inst Organ Chem, Siberian Branch, Ctr Spectral Invest, Novosibirsk 630090, Russia
5	(Данные Web of science) Siberian State Med Univ, Dept Morphol & Gen Pathol, Tomsk 634050, Russia
6	(Данные Web of science) Immanuel Kant Baltic Fed Univ, Basic Lab Immunol & Cell Biotechnol, Kaliningrad 236041, Russia
7	(Данные Web of science) Tomsk Polytech Univ, Res Sch Chem & Appl Biomed Sci, Tomsk 634050, Russia

4-oxo-4H-pyran-2.6-dicarboxylic acid (chelidonic acid, ChA) in the native state and in the complex with calcium [Ca(ChA)(H2O)(3)], named saucalchelin (CaChA), was isolated from the extract of Saussurea controversa leaves for the first time for the Asteraceae family. The structure of ChA was determined by NMR, MS and confirmed by X-ray analysis of its monomethyl ester, and CaChA was described by IR, ICP-MS, CHN analysis. The yield of ChA and CaChA was 45 mg/g and 70 mg/g of extract, respectively. The osteogenic activity of ChA, n-monobutyl ester of chelidonic acid, and CaChA has been studied in vitro in a 21-day culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in a standard nutrient medium without osteogenic supplements. CaChA significantly stimulated the growth of cell mass and differentiation of hAMMSCs into osteoblasts with subsequent mineralization of the culture and it may be a promising substance for accelerating bone tissue regeneration and engineering.

Avdeeva E. , Shults E. , Rybalova T. , Reshetov Y. , Porokhova E. , Sukhodolo I. , Litvinova L. , Shupletsova V. , Khaziakhmatova O. , Khlusov I. , Guryev A. , Belousov M.
Chelidonic Acid and Its Derivatives from Saussurea Controversa: Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro

Biomolecules. 2019. V.9. N5. 189 . DOI: 10.3390/biom9050189 WOS Scopus РИНЦ

Full text from publisher

Published online:

May 16, 2019

Web of science	WOS:000472671400029
Scopus	2-s2.0-85066234373
Elibrary	41629254
OpenAlex	W2945610555