Immobilized Bisphosphonates as Potential Inhibitors of Bioprosthetic Calcification: Effects on Various Xenogeneic Cardiovascular Tissues

Immobilized Bisphosphonates as Potential Inhibitors of Bioprosthetic Calcification: Effects on Various Xenogeneic Cardiovascular Tissues Full article

Journal

Biomedicines
ISSN: 2227-9059

Output data

Year: 2022, Volume: 10, Number: 1, Article number : 65, Pages count : DOI: 10.3390/biomedicines10010065

Tags

Bioprosthetic calcification; Bisphosphonate; Bovine jugular vein; Cross-linking; Diepoxide; Glutaraldehyde; Porcine aortic wall

Authors

Zhuravleva Irina Y. ¹ , Dokuchaeva Anna A. ¹ , Karpova Elena V. ² , Timchenko Tatyana P. ¹ , Titov Anatoly T. ³ , Shatskaya Svetlana S. ⁴ , Polienko Yuliya F. ²

Affiliations

1	E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya Street, Novosibirsk, 630055, Russian Federation
2	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentiev Avenue, Novosibirsk, 630090, Russian Federation
3	V. Sobolev Institute of Geology and Mineralogy SB RAS, 3 Academician Koptyug Avenue, Novosibirsk, 630090, Russian Federation
4	Institute of Solid State Chemistry and Mechanochemistry SB RAS, 18 Kutateladze Street, Novosibirsk, 630128, Russian Federation

Calcification is the major factor limiting the clinical use of bioprostheses. It may be prevented by the immobilization of bisphosphonic compounds (BPs) on the biomaterial. In this study, we assessed the accumulation and structure of calcium phosphate deposits in collagen-rich bovine pericardium (Pe) and elastin-rich porcine aortic wall (Ao) and bovine jugular vein wall (Ve) cross-linked with glutaraldehyde (GA) or diepoxy compound (DE). These tissues were then modified with pamidronic (PAM) acid or 2-(2′-carboxyethylamino)ethylidene-1,1-bisphosphonic (CEABA) acid. Tissue transformations were studied using Fourier-transform infrared spectroscopy. After subcutaneous implantation of the biomaterials in 220 rats, calcification dynamics were examined using atomic absorption spectrophotometry, light microscopy after von Kossa staining, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy The calcium content in all GA-cross-linked tissues and DE-cross-linked Ao increased to 100–160 mg/g on day 60 after implantation. BPs prevented the accumulation of phosphates on the surface of all materials and most effectively inhibited calcification in GA-cross-linked Ao and DE-cross-linked Pe. PAM containing-OH in the R1 group was more effective than CEABA containing-H in R1. The calcification-inhibitory effect of BPs may be realized through their ability to block nucleation and prevent the growth of hydroxyapatite crystals. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Zhuravleva I.Y. , Dokuchaeva A.A. , Karpova E.V. , Timchenko T.P. , Titov A.T. , Shatskaya S.S. , Polienko Y.F.
Immobilized Bisphosphonates as Potential Inhibitors of Bioprosthetic Calcification: Effects on Various Xenogeneic Cardiovascular Tissues
Biomedicines. 2022. V.10. N1. 65 . DOI: 10.3390/biomedicines10010065 WOS Scopus РИНЦ

Submitted:	Dec 1, 2021
Accepted:	Dec 27, 2021
Published print:	Dec 29, 2021
Published online:	Dec 29, 2021

Web of science	WOS:000750575100001
Scopus	2-s2.0-85122266928
Elibrary	47551108
OpenAlex	W4200310893