WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley

WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596

Output data

Year: 2023, Volume: 24, Number: 7, Pages: 6762 Pages count : 1 DOI: 10.3390/ijms24076762

Tags

CRISPR-associated endonculeases; cuticle; gene cluster; lipids; targeted mutagenesis; transcription factor; wax

Authors

Gerasimova Sophia V. ¹ , Kolosovskaya Ekaterina V. ² , Vikhorev Alexander V. ² , Korotkova Anna M. ¹ , Hertig Christian W. ³ , Genaev Mikhail A. ¹ , Domrachev Dmitry V. ⁴ , Morozov Sergey V. ⁴ , Chernyak Elena I. ⁴ , Shmakov Nikolay A. ¹ , Vasiliev Gennady V. ¹ , Kochetov Alex V. ¹ , Kumlehn Jochen ³ , Khlestkina Elena K. ²

Affiliations

1	Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
2	Vavilov Institute of General Genetics
3	Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Germany, Gatersleben
4	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences

Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the Arabidopsis thaliana AtWIN1 gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the HvWIN1 gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for win1 knockout (KO) and cer-x mutants showed that Cer-X and WIN1 are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of win1 KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known Cer-cqu locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the Cer-cqu gene cluster.

Gerasimova S.V. , Kolosovskaya E.V. , Vikhorev A.V. , Korotkova A.M. , Hertig C.W. , Genaev M.A. , Domrachev D.V. , Morozov S.V. , Chernyak E.I. , Shmakov N.A. , Vasiliev G.V. , Kochetov A.V. , Kumlehn J. , Khlestkina E.K.
WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley
International Journal of Molecular Sciences. 2023. V.24. N7. P.6762. DOI: 10.3390/ijms24076762 WOS РИНЦ

Submitted:	Feb 21, 2023
Accepted:	Mar 27, 2023
Published print:	Apr 5, 2023

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