Soloxolone methyl induces apoptosis and oxidative/ER stress in breast cancer cells and target cancer stem cell population

Soloxolone methyl induces apoptosis and oxidative/ER stress in breast cancer cells and target cancer stem cell population Full article

Journal

Turkish Journal of Biology
ISSN: 1300-0152

Output data

Year: 2023, Volume: 47, Number: 4, Pages: 247-261 Pages count : 17 DOI: 10.55730/1300-0152.2660

Tags

Mammosphere, soloxolone methyl, apoptosis, breast cancer

Authors

ERTÜRK ELİF ¹ , Oğuzhan AKGÜN ² , YILDIZ Yaren ² , ALPER KALKAN Pınar ^2,3 , Oksana V. SALOMATINA ⁴ , Nariman F. SALAKHUTDINOV ⁴ , Engin ULUKAYA ⁵ , Ferda ARI ²

Affiliations

1	Vocational School of Health Services
2	Department of Biology, Faculty of Science and Arts, Bursa Uludağ University
3	Istanbul University
4	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences
5	Department of Clinical Biochemistry, Faculty of Medicine, İstinye University

One of the most prevalent malignancies in women and one of the leading causes of cancer-related death is breast cancer. There is a need for new treatment approaches and drugs for breast cancer. Many studies show the high potential of triterpene compounds and their semisynthetic derivatives as anticancer agents due to their ability to induce apoptosis and suppress tumorigenesis. The effects of soloxolone methyl (SM), a semisynthetic derivative of 18-H-glycyrrhetinic acid, on the cytotoxicity and apoptosis of human breast cancer cell line (T-47D) and cancer stem cell (CSCs) population (mammospheres; CD44+/CD24-antigen) derived from breast cancer cells, were examined in this work. The ATP assay was used to determine SM growth-inhibitory effects. Fluorescent staining, caspase-cleaved cytokeratin 18, and flow cytometry analysis were used to determine the mode of the cell death. In addition, cell death was investigated at protein and gene levels by Western Blotting and PCR, respectively. SM resulted in cytotoxicity in a time and dose dependent manner via ROS production and ER stress in T-47D cells in 2 models. The mode of cell death was apoptosis, evidenced by phosphatidylserine exposure, caspase activation, and bax overexpression. In mammospheres as 3D model, SM decreased stem cell properties and induced cell death. Taken together, SM may be a promising agent in the treatment of breast cancer, especially due to its antigrowth activity on CSCs

ERTÜRK E. , Oğuzhan AKGÜN , YILDIZ Y. , ALPER KALKAN P. , Oksana V. SALOMATINA , Nariman F. SALAKHUTDINOV , Engin ULUKAYA , Ferda ARI
Soloxolone methyl induces apoptosis and oxidative/ER stress in breast cancer cells and target cancer stem cell population
Turkish Journal of Biology. 2023. Т.47. №4. С.247-261. DOI: 10.55730/1300-0152.2660 WOS Scopus РИНЦ

Submitted:	Oct 3, 2022
Accepted:	Jun 5, 2023
Published online:	Aug 31, 2023
Published print:	Oct 8, 2023

Web of science	WOS:001085260400003
Scopus	2-s2.0-85171863352
Elibrary	64667273
OpenAlex	W4386738749