The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming

The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming Full article

Journal

Journal of immunology research
ISSN: 2314-7156

Output data

Year: 2024, Volume: 2024, Article number : 7484490, Pages count : DOI: 10.1155/2024/7484490

Authors

Ruzanova Vera S. ¹ , Kirikovich Svetlana S. ¹ , Levites Evgeniy V. ¹ , Proskurina Anastasia S. ¹ , Dolgova Evgeniya V. ¹ , Ritter Genrikh S. ¹ , Efremov Yaroslav R. ^1,2 , Dubatolova Tatyana D. ³ , Sysoev Alexander V. ⁴ , Koleno Danil I. ⁴ , Ostanin Alexandr A. ⁵ , Chernykh Elena R. ⁵ , Bogachev Sergey S. ¹

Affiliations

1	Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
2	Novosibirsk National Research State University
3	Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences
4	N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences
5	Research Institute of Fundamental and Clinical Immunology

Macrophages are the immune cells of high-immunological plasticity, which can exert both pro- and anti-inflammatory activity, as well as repolarize their phenotype to the opposite or neutral one. In this regard, M2 macrophages of the tumor-associated stroma (TAS) are a promising therapeutic target in treating malignant neoplasms. Using FACS assay, we have estimated the CD11b+/Ly-6G+/Ly-6C+ fraction of macrophages from the peritoneum and TAS in intact healthy mice and those with developed Lewis carcinoma, both untreated and treated according to Karanahan technology in combination with group-specific macrophage activator (GcMAF-RF). As well, the pattern of pro- and anti-inflammatory cytokines mRNA expression in different groups of experimental and tumor-bearing animals was assessed. It was found that: (i) exposure of intact mice to GcMAF-RF results in the increased number of CD11b+/Ly-6C+ peritoneal macrophages and, at the same time, the expression pattern of cytokines in peritoneal macrophages switches from that characteristic of the mixed M1/M2 phenotype to that characteristic of the neutral M0 one; (ii) combination of Karanahan technology and GcMAF-RF treatment results in M0/M1 repolarization of TAS macrophages; (iii) in tumor-bearing mice, the response of peritoneal macrophages to such a treatment is associated with the induction of anti-inflammatory reaction, which is opposite to that in TAS macrophages.

Ruzanova V.S. , Kirikovich S.S. , Levites E.V. , Proskurina A.S. , Dolgova E.V. , Ritter G.S. , Efremov Y.R. , Dubatolova T.D. , Sysoev A.V. , Koleno D.I. , Ostanin A.A. , Chernykh E.R. , Bogachev S.S.
The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming
Journal of immunology research. 2024. V.2024. 7484490 . DOI: 10.1155/2024/7484490 WOS Scopus

Submitted:	Jun 7, 2023
Accepted:	Feb 19, 2024
Published online:	Feb 29, 2024

Web of science	WOS:001180482400002
Scopus	2-s2.0-85187523661
OpenAlex	W4392298341