Production of superoxide in chloroplast thylakoid membranes ESR study with cyclic hydroxylamines of different lipophilicity

Production of superoxide in chloroplast thylakoid membranes ESR study with cyclic hydroxylamines of different lipophilicity Full article

Journal

FEBS Letters
ISSN: 0014-5793

Output data

Year: 2011, Volume: 585, Number: 7, Pages: 1067-1071 Pages count : DOI: 10.1016/j.febslet.2011.03.004

Tags

Chloroplast; Electron transport chain; Oxygen reduction; Superoxide; Superoxide detector; Cyclic hydroxylamine; Electron spin resonance

Authors

Kozuleva Marina ¹ , Klenina Irina ¹ , Proskuryakov Ivan ¹ , Kirilyuk Igor ² , Ivanov Boris ¹

Affiliations

1	(Данные Web of science) Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia
2	(Данные Web of science) Russian Acad Sci, Novosibirsk Organ Chem Inst, Siberian Branch, Novosibirsk 630090, Russia

Accumulation of nitroxide radicals, DCP center dot or TMT center dot, under illumination of a thylakoid suspension containing either hydrophilic, DCP-H, or lipophilic, TMT-H, cyclic hydroxylamines that have high rate constants of the reaction with superoxide radicals, was measured using ESR. A slower accumulation of TMT center dot in contrast with DCP center dot accumulation was explained by re-reduction of TMT center dot by the carriers of the photosynthetic electron transport chain within the membrane. Superoxide dismutase suppressed TMT center dot accumulation to a lesser extent than DCP center dot accumulation. The data are interpreted as evidencing the production of intramembrane superoxide in thylakoids. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Kozuleva M. , Klenina I. , Proskuryakov I. , Kirilyuk I. , Ivanov B.
Production of superoxide in chloroplast thylakoid membranes ESR study with cyclic hydroxylamines of different lipophilicity
FEBS Letters. 2011. V.585. N7. P.1067-1071. DOI: 10.1016/j.febslet.2011.03.004 WOS Scopus

Published online:	Mar 4, 2011
Published print:	Apr 6, 2011

Web of science	WOS:000289057800021
Scopus	2-s2.0-79953253687
OpenAlex	W2150592608