Abstract: We present what is, to our knowledge, a new methodology for high-resolution three-dimensional imaging of oxygen concentration near live cells. The cells are placed in the buffer solution of a stable paramagnetic probe, and electron spin-resonance microimaging is employed to map out the probe's spin-spin relaxation time (T-2). This information is directly linked to the concentration of the oxygen molecule. The method is demonstrated with a test sample and with a small amount of live photosynthetic cells (cyanobacteria), under conditions of darkness and light. Spatial resolution of similar to 30 x 30 x 100 Am is demonstrated, with similar to mu M oxygen concentration sensitivity and sub-fmol absolute oxygen sensitivity per voxel. The use of electron spin-resonance microimaging for oxygen mapping near cells complements the currently available techniques based on micro-electrodes or fluorescence/phosphorescence. Furthermore, with the proper paramagnetic probe, it will also be readily applicable for intracellular oxygen microimaging, a capability which other methods find very difficult to achieve.

Cite: Halevy R. , Tormyshev V. , Blank A.
Microimaging of Oxygen Concentration near Live Photosynthetic Cells by Electron Spin Resonance
Biophysical Journal. 2010. V.99. N3. P.971-978. DOI: 10.1016/j.bpj.2010.05.002 WOS Scopus РИНЦ

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Dates:

Published print:

Aug 1, 2010

Identifiers:

Web of science	WOS:000280693100034
Scopus	2-s2.0-77955706570
Elibrary	15336807
OpenAlex	W2101270641

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