Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel

Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel Full article

Journal

Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024

Output data

Year: 2010, Volume: 46, Number: 7, Pages: 697-705 Pages count : DOI: 10.1134/S0003683810070045

Tags

Arthobacter nicotianae cells immobilized inside SiO2-xerogel; glucose/fructose isomerization; a plug-flow packed-bed reactor

Authors

Kovalenko G. A. ^1,2 , Perminova L. V. ¹ , Chernyak E. I. ³ , Sapunova L. I. ⁴

Affiliations

1	(Данные Web of science) Russian Acad Sci, Inst Catalysis, Siberian Branch, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
3	(Данные Web of science) Russian Acad Sci, Inst Organ Chem, Siberian Branch, Novosibirsk 630090, Russia
4	(Данные Web of science) Natl Acad Sci Belarus, Inst Microbiol, Minsk 220141, BELARUS

Macrokinetic peculiarites of heterogeneous process of monosaccharide (glucose/fructose) isomerization using biocatalysts prepared by incorporation of non-growing cells of a glucose isomerase-producing strain Arthobacter nicotianae inside SiO2-xerogel have been investigated. It was shown that the process proceeds in kinetic regime without diffusion limitation and biocatalyst activities at 60 and 75 degrees C were 19 and 32 U/g, respectively. Time of equilibrium in the reaction of monosaccharide isomerization was a function of starting ("triggering") glucose isomerase activity in a unit of reaction volume. When the activity exceeds 10 U/ml, equilibrium equimolar mixture of glucose and fructose was produced within a few hours. It was established that a continuous process carried out in a plug-flow packed-bed reactor is more efficient than a batch process accompanied with recycling, first of all, to significant improvement of operation stability of the designed biocatalysts. Under model conditions of industrial heterogeneous process of producing glucose-fructose syrups, the half-life time of inactivation of the biocatalysts was more than 500 h at (65 +/- 5)degrees C.

Kovalenko G.A. , Perminova L.V. , Chernyak E.I. , Sapunova L.I.
Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel
Applied Biochemistry and Microbiology. 2010. V.46. N7. P.697-705. DOI: 10.1134/S0003683810070045 WOS Scopus РИНЦ

Коваленко Г.А. , Перминова Л.В. , Черняк Е.И. , Сапунова Л.И.
Исследование макрокинетики гетерогенного процесса изомеризации моносахаридов с участием не растущих клеток продуцента глюкозоизомеразы arthrobacter nicotianae, иммобилизованных в sio 2 - ксерогеле
Биотехнология. 2009. №5. С.63-73.. РИНЦ

Published online:	Nov 3, 2010
Published print:	Dec 1, 2010

Web of science	WOS:000284166300004
Scopus	2-s2.0-78649310808
Elibrary	16618768
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