Mechanistic studies of the reactions of nitrone spin trap PBN with glutathiyl radical

Mechanistic studies of the reactions of nitrone spin trap PBN with glutathiyl radical Full article

Journal

Journal of Physical Chemistry B
ISSN: 1520-6106

Output data

Year: 2008, Volume: 112, Number: 15, Pages: 4841-4847 Pages count : 7 DOI: 10.1021/jp711548x

Authors

Polovyanenko Dmitriy N. , Plyusnin Victor F. , Reznikov Vladimir A. , Khramtsov Valery V. , Bagryanskaya Elena G.

Affiliations

1	(Данные Web of science) RAS, Int Tomog Ctr SB, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk Chem Kinet & Combust Inst, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
4	(Данные Web of science) RAS, Novosibirsk Organ Chem Inst SB, Novosibirsk 630090, Russia
5	(Данные Web of science) Ohio State Univ, Dorothy M Davis Heart & Lung Res Inst, Columbus, OH 43210 USA

We performed mechanistic studies of the reaction of PBN with the physiologically relevant glutathiyl radical, GS(center dot), formed upon oxidation of the intracellular antioxidant, glutathione, GSH. The scavenging rate constant of GS(center dot) by PBN has been measured directly by laser flash photolysis and indirectly by competitive EPR of the spin adduct of PBN and another spin trap, DMPO (5,5-dimethyl-1-pyrroline N-oxide), and was found to be 6.7 X 10(7) M-1 s(-1). Reverse decomposition of the paramagnetic PBN-glutathiyl radical adduct to the nitrone and thiyl radical was observed for the first time. The rate constant for the reaction of the monomolecular decomposition of the radical adduct was found to be 1.7 s(-1). Diamagnetic, EPR-invisible products of PBN adduct degradation were studied by H-1 NMR and F-19 NMR using newly synthesized fluorine-substituted PBN.

Polovyanenko D.N. , Plyusnin V.F. , Reznikov V.A. , Khramtsov V.V. , Bagryanskaya E.G.
Mechanistic studies of the reactions of nitrone spin trap PBN with glutathiyl radical
Journal of Physical Chemistry B. 2008. V.112. N15. P.4841-4847. DOI: 10.1021/jp711548x WOS Scopus РИНЦ

Published online:	Mar 26, 2008
Published print:	Apr 1, 2008

Web of science	WOS:000254883500049
Scopus	2-s2.0-45549088774
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