Glucose isomerase activity in suspensions of Arthrobacter nicotianae cells and adsorption immobilization of the microorganisms on inorganic carriers

Glucose isomerase activity in suspensions of Arthrobacter nicotianae cells and adsorption immobilization of the microorganisms on inorganic carriers Full article

Journal

Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024

Output data

Year: 2008, Volume: 44, Number: 2, Pages: 174-181 Pages count : 8 DOI: 10.1134/S0003683808020087

Authors

Kovalenko G.A. ¹ , Perminova L.V. ¹ , Terent'eva T.G. ¹ , Sapunova L.I. ¹ , Lobanok A.G. ¹ , Chuenko T.V. ² , Rudina N.A. ² , Chernyak E.I. ³

Affiliations

1	(Данные Web of science) Russian Acad Sci, Inst Catalysis, Siberian Div, Novosibirsk 630090, Russia
2	(Данные Web of science) Natl Acad Sci Byelarus, Inst Microbiol, Minsk 220141, BELARUS
3	(Данные Web of science) Russian Acad Sci, Siberian Div, Novosibirsk Organ Chem Inst, Novosibirsk 630090, Russia

Kinetics of monosaccharide isomerization has been studied in suspensions of intact, non-growing Arthrobacter nicotianae cells. Under the conditions of the study, glucose and fructose were isomerized at the same maximum rate of 700 mu mol/min per 1 g dried cells, which increased with temperature (the dependence was linear at 60-80 degrees C). The proposed means of adsorption immobilization of A. nicotianae cells involve inorganic carriers differing in macrostructure, chemical nature, and surface characteristics. Biocatalysts obtained by adsorbing the cells of A. nicotianae on carbon-containing foamed ceramics in the coarse of submerged cultivation were relatively stable and retained original activity (catalysis of monosaccharide isomerization) throughout 14 h of use at 70 degrees C. Maximum glucose isomerase activity (2 mu mol/min per 1 g) was observed with biocatalysts prepared by adsorption of non-growing A. nicotianae cells to the macroporous carbon-mineral carrier Sapropel and subsequent drying of the cell suspension together with the carrier.

Kovalenko G.A. , Perminova L.V. , Terent'eva T.G. , Sapunova L.I. , Lobanok A.G. , Chuenko T.V. , Rudina N.A. , Chernyak E.I.
Glucose isomerase activity in suspensions of Arthrobacter nicotianae cells and adsorption immobilization of the microorganisms on inorganic carriers
Applied Biochemistry and Microbiology. 2008. V.44. N2. P.174-181. DOI: 10.1134/S0003683808020087 WOS Scopus РИНЦ

Коваленко Г.А. , Перминова Л.В. , Терентьева Т.Г. , Сапунова Л.И. , Лобанок А.Г. , Чуенко Т.В. , Рудина Н.А. , Черняк Е.И.
Активность глюкозоизомеразы в суспензии клеток arthrobacter nicotianae и адсорбционная иммобилизация микроорганизмов на неорганических носителях
Прикладная биохимия и микробиология. 2008. Т.44. №2. С.193-201. РИНЦ

Published print:	Mar 1, 2008
Published online:	Jan 19, 2011

Web of science	WOS:000254701900008
Scopus	2-s2.0-41749101453
Elibrary	13580714
OpenAlex	W1988621400