Comparison of dried matrix spots and fabric phase sorptive extraction methods for quantification of highly potent analgesic activity agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma using LC-MS/MS

Comparison of dried matrix spots and fabric phase sorptive extraction methods for quantification of highly potent analgesic activity agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma using LC-MS/MS Full article

Journal

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN: 1570-0232 , E-ISSN: 1873-376X

Output data

Year: 2019, Volume: 1132, Article number : 121813, Pages count : DOI: 10.1016/j.jchromb.2019.121813

Tags

(2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol; Analgesic agent; Dried matrix spots; Fabric phase sorptive extraction; LC-MS/MS; FPSE biofluid sampler

Authors

Lastovka Anastasiya V. ^1,2 , Rogachev Artem D. ^1,2 , Il'ina Irina V. ² , Kabir Abuzar ³ , Volcho Konstantin P. ^1,2 , Fadeeva Valentina P. ^1,2 , Pokrovsky Andrey G. ² , Furton Kenneth G. ³ , Salakhutdinov Nariman F. ^1,2

Affiliations

1	(Данные Web of science) Russian Acad Sci, Siberian Branch, NN Vorozhtsov Novosibirsk Inst Organ Chem, Lavrentev Ave 9, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Pirogov St 2, Novosibirsk 630090, Russia
3	(Данные Web of science) Florida Int Univ, Int Forens Res Inst, Dept Chem & Biochem, 11200 SW 8th St, Miami, FL 33199 USA

The methods for quantification of highly potent analgesic agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl) octahydro-2H-chromen-4-ol in rat whole blood and plasma were developed and validated using dried matrix spots (DMS) or fabric phase sorptive extraction (FPSE) techniques in combination with LC-MS/MS. 2-Adamantylamine hydrochloride was used as an internal standard (IS). Chromatographic separation was carried out on a reversed-phase column (2.0 x 75 mm, 5 mu m) using water containing 0.1% formic acid and methanol containing 0.1% formic acid as mobile phases in gradient mode at a flow rate of 200 mu L/min. The mass spectrometric detection was performed using electrospray ionization (ESI) in positive ion mode. MRM transitions were m/z 284.5 -> 137.2/157.4 for the analgesic agent and m/z 152.3 -> 93.1/107.2 for IS. Calibration curves were linear within 20-5000 ng/mL in dried plasma spots (DPS) or dried blood spots (DBS) experiments. The linearity was obtained in the range of 20-5000 ng/mL and 50-5000 ng/mL for plasma-FPSE and blood-FPSE experiments, respectively. The intra- and inter-day accuracy and precision did not exceed acceptable limits. The mean extraction recovery (%) was 26 for DPS, 25 for DBS, 38 for plasma-FPSE, 31 for blood-FPSE.

Lastovka A.V. , Rogachev A.D. , Il'ina I.V. , Kabir A. , Volcho K.P. , Fadeeva V.P. , Pokrovsky A.G. , Furton K.G. , Salakhutdinov N.F.
Comparison of dried matrix spots and fabric phase sorptive extraction methods for quantification of highly potent analgesic activity agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma using LC-MS/MS
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2019. V.1132. 121813 . DOI: 10.1016/j.jchromb.2019.121813 WOS Scopus РИНЦ

Published print:

Nov 1, 2019

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