Abstract: A new potentially antioxidant compound, spin-labelled lutein (SL-lut), was synthesized and incorporated into egg yolk phosphatidylcholine (EYPC) liposome membrane. The approximate location of nitroxide free radical groups of SL-lut was determined based on electron paramagnetic resonance (EPR) spectra. Then the ability of SL-lut to protect EYPC liposomes against lipid peroxidation (LPO) was compared to the antioxidant effects of lutein and a nitroxide spin label 3-carbamoyl-2,2,5,5-tetramethylpyrrolidin- 1-yloxy (3-CP). Two free radical generation systems were used - a thermal decomposition of 2,2 '-azobis (2,4 dimethyl-valeronitrile) (AMVN) and a modified Fenton reaction using ferric-8-hydroxyquinoline (Fe( HQ) 3). Determination of the amount of thiobarbituric acid reactive species (TBARS) was used as a measure of LPO. SL-lut was the most powerful antioxidant, reducing LPO by about 6-times in AMVN-treated liposomes and 7-times in Fe(HQ)(3)-treated liposomes. Lutein alone gave only a moderate protection in both systems, while 3-CP was as efficient as SL-lut in the presence of AMVN, but not efficient whatsoever in the presence of Fe(HQ)(3). The results suggest that a nitroxide part of SL-lut plays an important role in enhancing the antioxidant activity of lutein and makes SL-lut a powerful antioxidant efficient under different conditions.

Cite: Broniowska K.A. , Kirilyuk I. , Wisniewska A.
Spin-labelled lutein as a new antioxidant in protection against lipid peroxidation
Free Radical Research. 2007. V.41. N9. P.1053-1060. DOI: 10.1080/10715760701463253 WOS Scopus РИНЦ

Dates:

Published print:	Jan 1, 2007
Published online:	Jul 7, 2009

Identifiers:

Web of science	WOS:000249958200015
Scopus	2-s2.0-34548329092
Elibrary	13562579
OpenAlex	W2129357093

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