Abstract: The ability of pure cultures of Rhodococcus actinobacteria from the Ural Specialized Collection of Alkanotrophic Microorganisms (World Federation for Culture Collections accession number 768; http://www.ecology.psu.ru/iegmcol) to convert beta-sitosterol (BSS) and its 3 beta-acylated derivatives was studied. Rhodococcus strains with pronounced cholesterol oxidase activity, capable of converting BSS to stigmat-4-ene-3-one in the reaction of cooxidation with n-hexadecane, were selected. The dependence of the activity of cholesterol oxidase of rhodococci on the length of the acyl group in BSS esters was studied. Conditions under which Rhodococcus cells convert BSS to 17 beta-hydroxyandrost-4-ene-3-one (testosterone), commonly used in pharmacology, were determined.

Cite: Ivshina I.B. , Grishko V.V. , Nogovitsina E.M. , Kukina T.P. , Tolstikov G.A.
Bioconversion of beta-sitosterol and its esters by actinobacteria of the genus Rhodococcus
Applied Biochemistry and Microbiology. 2005. V.41. N6. P.551-557. DOI: 10.1007/s10438-005-0100-y WOS Scopus РИНЦ

ArticleLinkType.TRANSLATED_TO_ORIGINAL: Ившина И.Б. , Гришко В.В. , Ноговицина Е.М. , Кукина Т.П. , Толстиков Г.А.
Биотрансформация β-ситостерола и его сложных эфиров актинобактериями рода RHODOCOCCUS
Прикладная биохимия и микробиология. 2005. Т.41. №6. РИНЦ

Dates:

Published print:

Nov 1, 2005

Identifiers:

Web of science	WOS:000233605300005
Scopus	2-s2.0-27744545876
Elibrary	13496650
OpenAlex	W2055198211

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