Quantitative determination of SH groups using F-19 NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid

Quantitative determination of SH groups using F-19 NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid Full article

Journal

Magnetic Resonance in Chemistry
ISSN: 0749-1581 , E-ISSN: 1097-458X

Output data

Year: 2005, Volume: 43, Number: 11, Pages: 902-909 Pages count : DOI: 10.1002/mrc.1652

Tags

NMR; thiols; thiol measurements; F-19; thiol-disulfide exchange; glutathione; fluorinated disulfides; benzoic acid

Authors

Potapenko DI , Bagryanskaya EG , Grigoriev IA , Maksimov AM , Reznikov VA , Platonov VE , Clanton TL , Khramtsov VV

Affiliations

1	(Данные Web of science) Ohio State Univ, Dorothy M Davis Heart & Lung Res Inst, Columbus, OH 43210 USA
2	(Данные Web of science) Int Tomog Ctr, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
4	(Данные Web of science) Inst Organ Chem, Novosibirsk 630090, Russia
5	(Данные Web of science) Inst Chem Kinet & Combust, Novosibirsk 630090, Russia

A new method of measurement of thiol concentration by F-19 NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid (BSSB). A significant broadening of the F-19 NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 +/- 11) X 10(3) M-1 s(-1) at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood. Copyright (c) 2005 John Wiley & Sons, Ltd.

Potapenko D. , Bagryanskaya E. , Grigoriev I. , Maksimov A. , Reznikov V. , Platonov V. , Clanton T. , Khramtsov V.
Quantitative determination of SH groups using F-19 NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid
Magnetic Resonance in Chemistry. 2005. V.43. N11. P.902-909. DOI: 10.1002/mrc.1652 WOS Scopus РИНЦ

Published print:

Nov 1, 2005

Web of science	WOS:000232943800003
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