F-19 NMR measurements of NO production in hypertensive ISIAH and OXYS rats

F-19 NMR measurements of NO production in hypertensive ISIAH and OXYS rats Full article

Journal

Biochemical and Biophysical Research Communications
ISSN: 0006-291X , E-ISSN: 1090-2104

Output data

Year: 2005, Volume: 330, Number: 2, Pages: 367-370 Pages count : DOI: 10.1016/j.bbrc.2005.02.166

Tags

nitric oxide; NO scavenging; F-19 NMR spin-trapping; nitronyl nitroxides; fluorinated probes; hypertensive rats; free radicals

Authors

Bobko AA , Sergeeva SV , Bagryanskaya EG , Markel AL , Khramtsov VV , Reznikov VA , Kolosova NG

Affiliations

1	(Данные Web of science) Inst Chem Kinet & Combust, Novosibirsk 630090, Russia
2	(Данные Web of science) Inst Cytol & Genet, Novosibirsk 630090, Russia
3	(Данные Web of science) Int Tomog Ctr, Novosibirsk 630090, Russia
4	(Данные Web of science) Inst Organ Chem, Novosibirsk 630090, Russia
5	(Данные Web of science) Ohio State Univ, Dorothy M Davis Heart & Lung Res Inst, Columbus, OH 43210 USA

Recently we demonstrated the principal possibility of application of F-19 NMR spin-trapping technique for in vivo (NO)-N-center dot detection [Free Radic. Biol. Med. 36 (2004) 248]. In the present Study, we employed this method to elucidate the significance of (NO)-N-center dot availability in animal models of hypertension. In vivo (NO)-N-center dot-induced conversion of the hydroxylamine of the fluorinated nitronyl nitroxide (HNN) to the hydroxylamine of the iminonitroxide (HIN) in hypertensive ISIAH and OXYS rat strains and normotensive Wistar rat strain was measured. Significantly lower HIN/HNN ratios were measured in the blood of the hypertensive rats. The NMR data were found to positively correlate with the levels of nitrite/nitrate evaluated by Griess method and negatively correlate with the blood pressure. In comparison with other traditionally used methods 19F NMR spectroscopy allows in vivo evaluation of (NO)-N-center dot production and provides the basis for in vivo (NO)-N-center dot imaging. (c) 2005 Elsevier Inc. All rights reserved.

Bobko A. , Sergeeva S. , Bagryanskaya E. , Markel A. , Khramtsov V. , Reznikov V. , Kolosova N.
F-19 NMR measurements of NO production in hypertensive ISIAH and OXYS rats
Biochemical and Biophysical Research Communications. 2005. V.330. N2. P.367-370. DOI: 10.1016/j.bbrc.2005.02.166 WOS Scopus РИНЦ

Published print:

May 1, 2005

Web of science	WOS:000228315800003
Scopus	2-s2.0-15744389618
Elibrary	13488645
OpenAlex	W2076241941