Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe

Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe Full article

Journal

JOURNAL OF MEMBRANE BIOLOGY
ISSN: 0022-2631 , E-ISSN: 1432-1424

Output data

Year: 2020, Volume: 253, Number: 4, Pages: 343-356 Pages count : 14 DOI: 10.1007/s00232-020-00132-3

Tags

Membrane potential; Thymocytes; Glycyrrhizic acid; Arabinogalactan; Drug delivery

Authors

Glazachev Yuri, I ¹ , Schlotgauer Anna A. ¹ , Timoshnikov Viktor A. ¹ , Kononova Polina A. ¹ , Selyutina Olga Yu ¹ , Shelepova Ekaterina A. ¹ , Zelikman Maxim, V ^1,4 , Khvostov Mikhail, V ^2,3 , Polyakov Nikolay E. ^1,4

Affiliations

1	(Данные Web of science) Inst Chem Kinet & Combust, Novosibirsk, Russia
2	(Данные Web of science) Novosibirsk State Univ, Novosibirsk, Russia
3	(Данные Web of science) Vorozhtsov Inst Organ Chem, Novosibirsk, Russia
4	(Данные Web of science) Inst Solid State Chem & Mechanochem, Novosibirsk, Russia

The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na(2)GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane. [GRAPHICS] .

Glazachev Y.I. , Schlotgauer A.A. , Timoshnikov V.A. , Kononova P.A. , Selyutina O.Y. , Shelepova E.A. , Zelikman M.V. , Khvostov M.V. , Polyakov N.E.
Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe
JOURNAL OF MEMBRANE BIOLOGY. 2020. V.253. N4. P.343-356. DOI: 10.1007/s00232-020-00132-3 WOS Scopus РИНЦ

Published online:	Jul 28, 2020
Published print:	Aug 1, 2020

Web of science	WOS:000553316900002
Scopus	2-s2.0-85088957806
Elibrary	45425995
OpenAlex	W3045656836