9H-Thioxanthen-9-one S,S-dioxide based redox active labels for electrochemical detection of DNA duplexes immobilized on Au electrodes

9H-Thioxanthen-9-one S,S-dioxide based redox active labels for electrochemical detection of DNA duplexes immobilized on Au electrodes Full article

Journal

Mendeleev Communications
ISSN: 0959-9436

Output data

Year: 2020, Volume: 30, Number: 3, Pages: 296-298 Pages count : 3 DOI: 10.1016/j.mencom.2020.05.011

Tags

thioxanthen-9-ones; redox active labels; electrochemical reduction; cyclic voltammetry; modified oligonucleotides; electrochemical genosensors

Authors

Shundrin Leonid A. ¹ , Os'kina Irina A. ¹ , Irtegova Irina G. ¹ , Poveshchenko Alexandr F. ²

Affiliations

1	(Данные Web of science) Russian Acad Sci, NN Vorozhtsov Novosibirsk Inst Organ Chem, Siberian Branch, Novosibirsk 630090, Russia
2	(Данные Web of science) Russian Acad Sci, Siberian Branch, Branch Inst Cytol & Genet, Res Inst Clin & Expt Lymphol, Novosibirsk 630117, Russia

The reaction of 2-bromomethyl-9H-thioxanthen-9-one with 2-/4-mercaptobenzoic acids led to 2-/4-[(9-oxo-9H-thioxanthen-2-yl)methylthio]benzoic acids. Their further oxidation by H2O2 gave the corresponding S,S-dioxides, which were used as redox active modifiers for a model oligonucleotide. Hybridization of the modified oligonucleotide with a complementary oligoprobe immobilized on Au electrode led to formation of the corresponding redox active DNA duplex, which was reliably detected by cyclic voltammetry.

Shundrin L.A. , Os'kina I.A. , Irtegova I.G. , Poveshchenko A.F.
9H-Thioxanthen-9-one S,S-dioxide based redox active labels for electrochemical detection of DNA duplexes immobilized on Au electrodes
Mendeleev Communications. 2020. V.30. N3. P.296-298. DOI: 10.1016/j.mencom.2020.05.011 WOS Scopus РИНЦ

Published print:

May 1, 2020

Web of science	WOS:000539369200011
Scopus	2-s2.0-85086110003
Elibrary	43296545
OpenAlex	W3033655185