Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side-Directed Spin Labeling of Proteins

Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side-Directed Spin Labeling of Proteins Full article

Journal

Chemistry - A European Journal
ISSN: 0947-6539 , E-ISSN: 1521-3765

Output data

Year: 2020, Volume: 26, Number: 12, Pages: 2705-2712 Pages count : 8 DOI: 10.1002/chem.201904587

Tags

EPR spectroscopy; human serum albumin; proteins; spin labeling; triarylmethyl radical

Authors

Tormyshev Victor M. ¹ , Chubarov Alexey S. ² , Krumkacheva Olesya A. ^1,3 , Trukhin Dmitry V. ¹ , Rogozhnikova Olga Yu. ¹ , Spitsyna Anna S. ¹ , Kuzhelev Andrey A. ¹ , Koval Vladimir V. ² , Fedin Matvey V. ³ , Godovikova Tatyana S. ² , Bowman Michael K. ⁴ , Bagryanskaya Elena G. ¹

Affiliations

1	(Данные Web of science) RAS, SB, NN Vorozhtsov Novosibirsk Inst Organ Chem, Pr Lavrentjeva 9, Novosibirsk 630090, Russia
2	(Данные Web of science) RAS, SB, Inst Chem Biol & Fundamental Med, Pr Lavrentjeva 8, Novosibirsk 630090, Russia
3	(Данные Web of science) RAS, SB, Int Tomog Ctr, Inst Skaya Str 3a, Novosibirsk 630090, Russia
4	(Данные Web of science) Univ Alabama, Dept Chem & Biochem, Tuscaloosa, AL 35487 USA

Trityl radicals (TAMs) have recently appeared as an alternative source of spin labels for measuring long distances in biological systems. Finland trityl radical (FTAM) served as the basis for this new generation of spin labels, but FTAM is rather lipophilic and susceptible to self-aggregation, noncovalent binding with lipophilic sites of proteins, and noncovalent docking at the termini of duplex DNA. In this paper the very hydrophilic OX063 TAM with very low toxicity and little tendency for aggregation is used as the basis for a spin label. Human serum albumin (HSA) labeled with OX063 has an intense narrow line typical of TAM radicals in solution, whereas HSA labeled with FTAM shows broad lines and extensive aggregation. In pulse EPR measurements, the measured phase memory time T-M for HSA labeled with OX063 is 6.3 mu s at 50 K, the longest yet obtained with a TAM-based spin label. The lowered lipophilicity also decreases side products in the labeling reaction.

Tormyshev V.M. , Chubarov A.S. , Krumkacheva O.A. , Trukhin D.V. , Rogozhnikova O.Y. , Spitsyna A.S. , Kuzhelev A.A. , Koval V.V. , Fedin M.V. , Godovikova T.S. , Bowman M.K. , Bagryanskaya E.G.
Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side-Directed Spin Labeling of Proteins
Chemistry - A European Journal. 2020. V.26. N12. P.2705-2712. DOI: 10.1002/chem.201904587 WOS Scopus РИНЦ

Published online:	Feb 6, 2020
Published print:	Feb 26, 2020

Web of science	WOS:000511258400001
Scopus	2-s2.0-85079217560
Elibrary	43238334
OpenAlex	W2994796880