Abstract: The spatial structure of duplex (Phn-NH(CH2)2NH)pd(CCAAACA).pd(TGTTTGGC) having a N-(2-oxyethyl)-phenazinium residue covalently linked with the 5'-terminal phosphate of the heptanucleotide was studied by means of one- and two-dimensional H-1-NMR spectroscopy. The resonances of phenazinium protons, ethylenediamine linker protons, as well as, oligonucleotide H5/H6/H8/CH3 base protons and H1', H2'a, H2'b, H3', H4' deoxyribose protons have been assigned by means of H-1-COSY, H-1-NOESY and H-1-C-13-COSY. The presence of the phenazine residue in duplex causes an additional imino proton signal of the terminal (G-7).(C-1) base pair, suggesting a higher stability of the duplex (Phn-NH(CH2)2NH)pd(CCAAACA).pd(TGTTTGGC) as compared to the unmodified duplex pd(CCAAACA).pd(TGTTTGGC). Analysis of NOE interactions between protons of the dye and the oligonucleotides show the phenazinium polycyclic system to intercalate between G-7 and C-8 residues of the octanucleotide.

Cite: Bichenkova E.V. , Zarytova V.F. , Ivanova E.M. , Maltseva T.V. , Salnicov G.E. , Lebedev A.V.
STRUCTURAL INVESTIGATION OF DUPLEX (PHN-NH(CH2)2NH)PD(CCAAACA)BULLET-PD(TGTTTGGC) HAVING COVALENTLY LINKED N-(2-OXYETHYL)-PHENAZINIUM RESIDUE BY MEANS OF 2D H-1-NMR SPECTROSCOPY IN AQUEOUS-SOLUTION
Биоорганическая химия (RUSS J BIOORG CHEM+). 1992. V.18. N3. P.398-412. WOS

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Web of science

WOS:A1992HW74300007