A study of tribendimidine effects in vitro and in vivo on the liver fluke Opisthorchis felineus

A study of tribendimidine effects in vitro and in vivo on the liver fluke Opisthorchis felineus Full article

Journal

Parasites and Vectors
ISSN: 1756-3305

Output data

Year: 2019, Volume: 12, Article number : 23, Pages count : DOI: 10.1186/s13071-019-3288-z

Tags

Food-borne trematodes; Opisthorchis felineus; Tribendimidine; in vivo; in vitro; Hamster model

Authors

Pakharukova Maria Y. ^1,2 , Samsonov Vladimir A. ³ , Serbina Elena A. ⁴ , Mordvinov Viatcheslav A. ¹

Affiliations

1	(Данные Web of science) SB RAS, Inst Cytol & Genet, 10 Lavrentiev Ave, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, 2 Pirogova Str, Novosibirsk 630090, Russia
3	(Данные Web of science) SB RAS, Vorozhtsov Inst Organ Chem, Novosibirsk, Russia
4	(Данные Web of science) SB RAS, Inst Systemat & Ecol Anim, Novosibirsk, Russia

BackgroundThe food-borne liver fluke Opisthorchis felineus is an epidemiologically important species and the causative agent of opisthorchiasis across an extensive territory of Eurasia. For decades, treatment of opisthorchiasis has been based on praziquantel. Tribendimidine could be an alternative drug that has been successfully tested for Opisthorchis viverrini and Clonorchis sinensis infections. We aimed to assess tribendimidine effects in comparison with praziquantel in vivo and in vitro against the liver fluke Opisthorchis felineus.ResultsIn this study we (i) calculated half-maximal inhibitory concentrations (IC50) by motility tests against O. felineus adults and newly excysted metacercarie after tribendimidine treatment in vitro; (ii) determined whether tribendimidine and PZQ effects on adult liver flukes are dependent on or mediated by white blood cells; and (iii) tested in vivo the anthelmintic activity of tribendimidine on juvenile and adult worms. We found that the efficiency of tribendimidine in vitro was similar (IC50 = 0.23 M for newly excysted metacercariae and 0.19 M for adult worms) to that of praziquantel (IC50 0.98 M for newly excysted metacercariae and 0.47 M for adult worms). The treatment of adult worms in vivo with praziquantel or tribendimidine at 400 mg/kg resulted in a 76% and 77.2% reduction, respectively, in the worm burden during chronic infection.ConclusionsThe differences between WBR values after PZQ and TBN treatment were not significant, thus tribendimidine was as effective as praziquantel against O. felineus liver flukes. Given the broad-spectrum activity of tribendimidine and efficacy against O. felineus, this drug may be a promising candidate for the treatment of opisthorchiasis felinea and other liver fluke infections.

Pakharukova M.Y. , Samsonov V.A. , Serbina E.A. , Mordvinov V.A.
A study of tribendimidine effects in vitro and in vivo on the liver fluke Opisthorchis felineus

Parasites and Vectors. 2019. V.12. 23 . DOI: 10.1186/s13071-019-3288-z WOS Scopus РИНЦ

Full text from publisher

Published online:	Jan 11, 2019
Published print:	Dec 1, 2019

Web of science	WOS:000455550800007
Scopus	2-s2.0-85059868562
Elibrary	38663443
OpenAlex	W2912148394