Effects of fluorine-containing usnic acid and fungus Beauveria bassiana on the survival and immune-physiological reactions of Colorado potato beetle larvae

Effects of fluorine-containing usnic acid and fungus Beauveria bassiana on the survival and immune-physiological reactions of Colorado potato beetle larvae Full article

Journal

Pest Management Science
ISSN: 1526-498X , E-ISSN: 1526-4998

Output data

Year: 2018, Volume: 74, Number: 3, Pages: 598-606 Pages count : 9 DOI: 10.1002/ps.4741

Tags

usnic acid; fluorine; Colorado potato beetle; Beauveria bassiana; immunity; synergy

Authors

Kryukov Vadim Yu ¹ , Tomilova Oksana G. ¹ , Luzina Olga A. ² , Yaroslavtseva Olga N. ¹ , Akhanaev Yuriy B. ¹ , Tyurin Maksim V. ¹ , Duisembekov Bahytzhan A. ³ , Salakhutdinov Nariman F. ² , Glupov Viktor V. ¹

Affiliations

1	(Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Systemat & Ecol Anim, Novosibirsk, Russia
2	(Данные Web of science) Russian Acad Sci, Siberian Branch, NN Vorozhtsov Novosibirsk Inst Organ Chem, Novosibirsk, Russia
3	(Данные Web of science) Kazakh Res Inst Plant Protect & Quarantine, Alma Ata, Kazakhstan

BACKGROUND: The search for compounds that interact synergistically with entomopathogenic fungi is aimed at enhancing the efficacy and stability of biological products against pest insects, for example, against the Colorado potato beetle (CPB). We hypothesized that fluorine-containing derivatives of usnic acid (FUA) might be candidates for the development of multicomponent bio-insecticides. The aim of this study was to analyze the co-influence of FUA and Beauveria bassiana on the survival and immune-physiological reactions of CPB larvae. RESULTS: Synergy between FUA and B. bassiana was observed after treatment of second, third and fourth larvae instars under laboratory conditions. Furthermore, synergy was observed in field trials in continental climate conditions in southeastern Kazakhstan. In a field experiment, the median lethal time was shortened three-fold, and cumulative mortality for 15 days increased by 36% in the combined treatment compared with a fungal infection alone. FUA treatment delayed larval development, decreased the total hemocyte count, and increased both the phenoloxidase activity in integuments and the detoxification enzyme rate in hemolymph. A combined treatment with fungus and FUA led to increases in the aforementioned changes. CONCLUSION: Toxicosis caused by FUA provides a stable synergistic effect between FUA and B. bassiana. The combination can be promising for the development of highly efficient products against CPB. (C) 2017 Society of Chemical Industry

Kryukov V.Y. , Tomilova O.G. , Luzina O.A. , Yaroslavtseva O.N. , Akhanaev Y.B. , Tyurin M.V. , Duisembekov B.A. , Salakhutdinov N.F. , Glupov V.V.
Effects of fluorine-containing usnic acid and fungus Beauveria bassiana on the survival and immune-physiological reactions of Colorado potato beetle larvae
Pest Management Science. 2018. V.74. N3. P.598-606. DOI: 10.1002/ps.4741 WOS Scopus

Published online:	Nov 2, 2017
Published print:	Mar 1, 2018

Web of science	WOS:000423777600012
Scopus	2-s2.0-85041293517
OpenAlex	W2758291403