A Versatile Approach to Attachment of Triarylmethyl Labels to DNA for Nanoscale Structural EPR Studies at Physiological Temperatures

A Versatile Approach to Attachment of Triarylmethyl Labels to DNA for Nanoscale Structural EPR Studies at Physiological Temperatures Full article

Journal

Journal of Physical Chemistry B
ISSN: 1520-6106

Output data

Year: 2018, Volume: 122, Number: 1, Pages: 137-143 Pages count : 7 DOI: 10.1021/acs.jpcb.7b10689

Authors

Shevelev Georgiy Yu. ^1,2 , Gulyak Evgeny L. ^1,2 , Lomzov Alexander A. ^1,2 , Kuzhelev Andrey A. ^2,4 , Krumkacheva Olesya A. ^2,3 , Kupryushkin Maxim S. ¹ , Tormyshev Victor M. ^2,4 , Fedin Matvey V. ^2,3 , Bagryanskaya Elena G. ^2,3,4 , Pyshnyi Dmitrii V. ^1,2

Affiliations

1	(Данные Web of science) Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
3	(Данные Web of science) Int Tomog Ctr SB RAS, Novosibirsk 630090, Russia
4	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, Novosibirsk 630090, Russia

Triarylmethyl (trityl, TAM) radicals are a promising class of spin labels for nanometer-scale distance measurements in biomolecules at physiological temperatures. However, to date, existing approaches to site-directed TAM labeling of DNA have been limited to label attachment at the termini of oligonucleotides, thus hindering a majority of demanded applications. Herein, we report a new versatile strategy for TAM attachment at arbitrary sites of nucleic acids. It utilizes an achiral non-nucleoside phosphoramidite monomer for automated solid-phase synthesis of oligonucleotides, which are then postsynthetically functionalized with TAM. We demonstrate a synthesis of a set of oligonucleotide complexes that are TAM-labeled at internal or terminal sites, as well as the possibility of measuring interspin distances up to similar to 5-6 nm at 298 K using double quantum coherence electron paramagnetic resonance (EPR). Implementation of the developed approach strongly broadens the scope of nucleic acids and nucleoprotein complexes available for nanoscale structural EPR studies at room temperatures.

Shevelev G.Y. , Gulyak E.L. , Lomzov A.A. , Kuzhelev A.A. , Krumkacheva O.A. , Kupryushkin M.S. , Tormyshev V.M. , Fedin M.V. , Bagryanskaya E.G. , Pyshnyi D.V.
A Versatile Approach to Attachment of Triarylmethyl Labels to DNA for Nanoscale Structural EPR Studies at Physiological Temperatures
Journal of Physical Chemistry B. 2018. V.122. N1. P.137-143. DOI: 10.1021/acs.jpcb.7b10689 WOS Scopus РИНЦ

Published online:	Dec 20, 2017
Published print:	Jan 11, 2018

Web of science	WOS:000422814000014
Scopus	2-s2.0-85040553706
Elibrary	35530965
OpenAlex	W2773609366