Towards optical control of single blood platelet activation

Towards optical control of single blood platelet activation Full article

Conference

SARATOV FALL MEETING 2017: LASER PHYSICS AND PHOTONICS XVIII; AND COMPUTATIONAL BIOPHYSICS AND ANALYSIS OF BIOMEDICAL DATA IV Saratov, 26–30 сентября 2017 года
26-30 Sep 2017 , Саратов

Journal

Proceedings of SPIE - The International Society for Optical Engineering
ISSN: 0277-786X , E-ISSN: 1996-756X

Output data

Year: 2018, Volume: 10717, Article number : 1071722, Pages count : DOI: 10.1117/12.2305477

Tags

platelet activation; caged epinephrine; single cell optical control; biophotonics

Authors

Spiryova Darya V. ¹ , Karmatskih Oleg Yu. ¹ , Vorob'ev Alexei Yu. ^1,2 , Moskalensky Alexander E. ^1,3

Affiliations

1	(Данные Web of science) Novosibirsk State Univ, Pirogova Str 2, Novosibirsk 630090, Russia
2	(Данные Web of science) SB RAS, NN Vorozhtsov Novosibirsk Inst Organ Chem, 9 Lavrentiev Ave, Novosibirsk 630090, Russia
3	(Данные Web of science) SB RAS, Voevodsky Inst Chem Kinet & Combust, Inst Skaya 3, Novosibirsk 630090, Russia

Blood platelets play a pivotal role in blood coagulation and in other normal and pathological processes. The understanding of fundamental mechanisms underlying their functions is very important for diagnostics and treatment. Single-cell experiments are needed for this purpose, which are complicated by insufficient spatiotemporal precision of conventional activation protocols. We present an approach to trigger single platelet activation optically, without the need of reagent mixing. This is achieved using photolabile compound, which rapidly delivers epinephrine upon UV irradiation. We demonstrated the applicability of the technique to rapidly induce platelet activation for studying dynamics of activation. The presented method may give novel fundamental knowledge about platelet functions and facilitate current research of their ability to deliver drugs to tumors or vascular injury sites.

Spiryova D.V. , Karmatskih O.Y. , Vorob'ev A.Y. , Moskalensky A.E.
Towards optical control of single blood platelet activation
Proceedings of SPIE - The International Society for Optical Engineering. 2018. V.10717. 1071722 . DOI: 10.1117/12.2305477 WOS Scopus РИНЦ

Published print:

Apr 26, 2018

Web of science	WOS:000451708400073
Scopus	2-s2.0-85047362362
Elibrary	35513683
OpenAlex	W2802898417