Microscale ion exchange chromatography and spectral analysis of oligonucleotides

Microscale ion exchange chromatography and spectral analysis of oligonucleotides Full article

Journal

Molecular Biology
ISSN: 0026-8933

Output data

Year: 1972, Volume: 6, Number: 6, Pages: 653-659 Pages count : 7

Authors

Vlasov V.V. ¹ , Grachev M.A. ¹ , Komarova N.I. ¹ , Kuz'min S.V. ¹ , Menzorova N.I. ¹

Affiliations

1	(Scopus) Inst. Org. Chem., Siberian Branch, Acad. Scis USSR, Novosibirsk, Russian Federation

Methods of column ion exchange chromatography of oligonucleotides were developed which, when combined with the use of a microspectrophotometer, allow the amount of substance necessary for analysis to be reduced by 100 to 1000 times as compared with the usual scale. The possibilities of the method were demonstrated on an example of separating and identifying the oligonucleotides of a pyrimidyl ribonuclease hydrolyzate of valine tRNA.

Vlasov V.V. , Grachev M.A. , Komarova N.I. , Kuz'min S.V. , Menzorova N.I.
Microscale ion exchange chromatography and spectral analysis of oligonucleotides
Molecular Biology. 1972. V.6. N6. P.653-659. Scopus РИНЦ

Scopus	2-s2.0-0015472105
Elibrary	30840166