Abstract: 1. The kinetics of the formation of guanosine 5′-triphosphate, 5′-diphosphate, and 5′-monophosphate in the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate in pyridine at 27° has been recorded. 2. In the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate, four successive reactions take place: those of the formation of an intermediate compound, of GTP, of GDP, and of GMP. The maximum formation of GTP (67%) occurs after 2 hours and of GDP (67%) after 24 hours. 3. A preparative method for obtaining pure tetrasodium guanosine 5′-triphosphate from the morpholide of guanosine 5′-monophosphate with its isolation from the mixture by chromatography on DEAE-cellulose in a linear gradient of triethylammonium bicarbonate has been developed. The yield calculated on the basis of the morpholide, is 61-65%. © 1966 Plenum Publishing Corporation.

Cite: Lomakina T.S. , Grineva N.I.
Preparative method of obtaining guanosine 5′-triphosphate
Chemistry of Natural Compounds. 1965. V.1. N4. P.213-219. DOI: 10.1007/BF00566872 Scopus РИНЦ

Dates:

Published print:

Jul 1, 1965

Identifiers:

Scopus	2-s2.0-34250517996
Elibrary	30925281
OpenAlex	W2020065320

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