A comparative study of the antiviral activity of chemical compounds concerning the orthopoxviruses experiments in vivo [Сравнительное изучение противовирусной активности химических соединений в отношении ортопоксвирусов в экспериментах IN VIVO]

A comparative study of the antiviral activity of chemical compounds concerning the orthopoxviruses experiments in vivo [Сравнительное изучение противовирусной активности химических соединений в отношении ортопоксвирусов в экспериментах IN VIVO] Full article

Journal

Вопросы вирусологии/Voprosy Virusologii
ISSN: 0507-4088

Output data

Year: 2013, Volume: 58, Number: 4, Pages: 39-43 Pages count : 5

Tags

5-dion; Antiviral activity; Condensed derivatives of pyr-rolidine; Ectromelia virus; Mice; Monkeypox virus; Orthopoxviruses

Authors

Kabanov A.S. ¹ , Sergeev Al.A. ¹ , Shishkina L.N. ¹ , Bulychev L.E. ¹ , Skarnovich M.O. ¹ , Sergeev At.A. ¹ , Bormotov N.I. ¹ , Pyankov O.V. ¹ , Serova O.A. ¹ , Bodnev S.A. ¹ , Selivanov B.A. ² , Tikhonov A.Ya. ² , Agafonov A.P. ¹ , Sergeev A.N. ²

Affiliations

1	(Scopus) State Research Center of Virology and Biotechnology Vector, 630559, Koltsovo, Novosibirsk Region, Russian Federation
2	(Scopus) Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russian Federation

In the experiments using intranasal (i/n) infection of mice with the ectromelia virus (EV) in a dose 10 LD50/head (10 x 50% lethal dose/head) or with the monkeypox virus (MPXV) in a dose 10 ID50/head (10 x 50% infective dose/ head) it was demonstrated that the antiviral efficiency of chemical compounds - the condensed derivatives of pyrrolidin-2,5-dion, as well as their predecessors and the nearest analogues, synthesized in Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences (NIOCH SB RAS) was observed. As a positive control we used the antipoxvirus chemical preparation ST-246 available from SIGA Technologies Inc. (USA), synthesized in NIOCH SB RAS by the technique suggested by the authors. It was demonstrated that the compound NIOCH-14 (7-[N'-(4-Trifluoromethylbenzoil)-hydrazidecarbonil]- tricyclo[3.2.2.02,4]non-S-en-6-carbonic acid) possessed comparable with ST-246 antiviral activity concerning EV and MPXV on all indicators used. Therefore, at infection of mice with EV (strain K-1) and peroral administration of NIOCH-14 and ST-246 in a dose 50 mkg/g of mouse weight (12-14 g) within 10 days the survival rate and average life expectancy of mice authentically exceeded the control levels. EV titers in lungs through 6 days after Infection in the same groups were lower than in the control. In addition to that, after 7 days of infection of mice with MPXV (strain V79-1-005) and daily peroral administration of NIOCH-14 and ST-246 In a dose 60 mkg/g of mouse weight (9-11 g) authentic decrease in a part of infected animals and MPXV titers in lungs was observed.

Kabanov A.S. , Sergeev A.A. , Shishkina L.N. , Bulychev L.E. , Skarnovich M.O. , Sergeev A.A. , Bormotov N.I. , Pyankov O.V. , Serova O.A. , Bodnev S.A. , Selivanov B.A. , Tikhonov A.Y. , Agafonov A.P. , Sergeev A.N.
A comparative study of the antiviral activity of chemical compounds concerning the orthopoxviruses experiments in vivo [Сравнительное изучение противовирусной активности химических соединений в отношении ортопоксвирусов в экспериментах IN VIVO]
Вопросы вирусологии/Voprosy Virusologii. 2013. Т.58. №4. С.39-43. Scopus РИНЦ

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