The morphofunctional and biochemical characteristics of opisthorchiasis-associated cholangiocarcinoma in a Syrian hamster model

The morphofunctional and biochemical characteristics of opisthorchiasis-associated cholangiocarcinoma in a Syrian hamster model Full article

Journal

Russian Journal of Genetics: Applied Research
ISSN: 2079-0597

Output data

Year: 2016, Volume: 6, Number: 4, Pages: 454-462 Pages count : 9 DOI: 10.1134/S2079059716040134

Tags

cholangiocarcinoma; liver; opisthorchiasis; Syrian hamsters Mesocricetus auratus

Authors

Maksimova G.A. ¹ , Pakharukova M.Y. ¹ , Kashina E.V. ¹ , Zhukova N.A. ² , Lvova M.N. ¹ , Khvostov M.V. ² , Baev D.S. ² , Katokhin A.V. ¹ , Tolstikova T.G. ² , Mordvinov V.A. ^1,3

Affiliations

1	(Scopus) Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russian Federation
2	(Scopus) Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russian Federation
3	(Scopus) Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation

The validity of the experimental models of pathologies is one of the key challenges in translational medicine. Cholangiocarcinoma, or bile duct cancer, ranks second among oncological diseases of the liver. There is a strong association between bile duct cancer and parasitic infestation of the liver caused by trematodes in the Opisthorchiidae family. We recently demonstrated that cholangiocarcinoma can develop in Syrian hamsters (Mesocricetus auratus) infected by Opisthorchis felineus and administered with dimethylnitrosamine. However, there is still no description of how this experimental model can be used in translational research. The aim of this work was to study the morphological, functional, and biochemical characteristics during cholangiocarcinoma development in Syrian hamsters infected by O. felineus and administered with dimethylnitrosamine. The experiment lasted 30 weeks with combined administration of dimethylnitrosamine in drinking water at a dose of 12.5 ppm and a single injection of 50 metacercariae O. felineus. It was shown that the development of cholangiocarcinoma (18 weeks) increased the total number of basophils, eosinophils, and monocytes; the relative number of granulocytes, as well as the amount of total and direct bilirubin; and the cholesterol and ALT levels; however, it reduced the relative number of lymphocytes. Based on pathological, morphometric, and biochemical analyses, our model has characteristics similar to those in patients with opisthorchiasis-associated cholangiocarcinoma. Thus, this model can be used to test anticancer drugs, to study the mechanisms of cholangiocarcinogenesis, and to search for molecular markers for early diagnosis of bile duct cancer. © 2016, Pleiades Publishing, Ltd.

Maksimova G.A. , Pakharukova M.Y. , Kashina E.V. , Zhukova N.A. , Lvova M.N. , Khvostov M.V. , Baev D.S. , Katokhin A.V. , Tolstikova T.G. , Mordvinov V.A.
The morphofunctional and biochemical characteristics of opisthorchiasis-associated cholangiocarcinoma in a Syrian hamster model
Russian Journal of Genetics: Applied Research. 2016. V.6. N4. P.454-462. DOI: 10.1134/S2079059716040134 Scopus РИНЦ

Published print:	Jun 1, 2016
Published online:	Jun 15, 2016

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