Development of assay method by HPLC-DAD for the quantitative determination of chromones in Saposhnikovia divaricata radices and its validation

Development of assay method by HPLC-DAD for the quantitative determination of chromones in Saposhnikovia divaricata radices and its validation Full article

Conference

The IX School-Seminar of Young Russian Scientists "Problems of Sustainable Regional Development", dedicated to the 70th anniversary of Academician of the Russian Academy of Sciences Arnold Kirillovich Tulokhonov 3–7 July 2019, Ulan-Ude, Russian Federation
03-07 Jul 2019 , Ulan-Ude

Journal

IOP Conference Series: Earth and Environmental Science
ISSN: 1755-1307

Output data

Year: 2019, Volume: 320, Number: 1, Article number : 012056, Pages count : DOI: 10.1088/1755-1315/320/1/012056

Authors

Urbagarova B.M. ^1,2 , Taraskin V.V. ² , Shul'ts E.E. ³ , Radnaeva L.D. ^1,2

Affiliations

1	(Scopus) Banzarov Buryat State University, Ulan-Ude, 670000, Russian Federation
2	(Scopus) Baikal Institute of Nature Management SB RAS, Ulan-Ude, 670047, Russian Federation
3	(Scopus) N N Vorozhtsov Novosibirsk Institute of Organic Chemistry, SB RAS, Novosibirsk, 630090, Russian Federation

Assay method using high performance liquid chromatography with diode array detection was developed and validated for the determination of chromones: prim-Oglucosylcimifugin, cimifugin, 4'-O-β-D-glucosyl-5-O-methylisamminol. Chromatographic method for the determination of chromones was validated for specificity, linearity, accuracy, repeatability and intermediate precision. The optimal conditions for the extraction of chromones from the Saposhnikoviae divaricatae radices were selected. It was found that these substances are better extracted with 50% ethanol using ultrasonic extraction at room temperature. The quantity of chromones in Saposhnikoviae Radix of the flora of Buryatia was also determined. © Published under licence by IOP Publishing Ltd.

Urbagarova B.M. , Taraskin V.V. , Shul'ts E.E. , Radnaeva L.D.
Development of assay method by HPLC-DAD for the quantitative determination of chromones in Saposhnikovia divaricata radices and its validation

IOP Conference Series: Earth and Environmental Science. 2019. V.320. N1. 012056 . DOI: 10.1088/1755-1315/320/1/012056 Scopus

Full text from publisher

Published online:

Aug 29, 2019

Scopus	2-s2.0-85073018680
OpenAlex	W2970515348