Optical uncaging of ADP reveals the early calcium dynamics in single, freely moving platelets

Optical uncaging of ADP reveals the early calcium dynamics in single, freely moving platelets Full article

Journal

Biomedical Optics Express
ISSN: 2156-7085

Output data

Year: 2020, Volume: 11, Number: 6, Pages: 3319-3329 Pages count : 11 DOI: 10.1364/BOE.392745

Authors

Spiryova D.V. ¹ , Vorobev A.Y. ^1,2 , Klimontov V.V. ^1,3 , Koroleva E.A. ^1,3 , Moskalensky A.E. ^1,4

Affiliations

1	(Scopus) Novosibirsk State University, Novosibirsk, 630090, Russian Federation
2	(Scopus) N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, SB, RAS, Novosibirsk, 630090, Russian Federation
3	(Scopus) Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630117, Russian Federation
4	(Scopus) Voevodsky Institute of Chemical Kinetics and Combustion, SB, RAS, Novosibirsk, 630090, Russian Federation

Platelet activation is considered to be a cornerstone in pathogenesis of cardiovascular disease. The assessment of platelet activation at the single-cell level is a promising approach for the research of platelet function in physiological and pathological conditions. Previous studies used the immobilization of platelets on the surface, which significantly alters the activation signaling. Here we show that the use of photolabile "caged" analog of ADP allows one to track the very early stage of platelet activation in single, freely moving cells. In this approach, the diffusion step and ADP receptor ligation are separated in time, and a millisecond-timescale optical pulse may trigger the activation. The technique allows us to measure the delay (lag time) between the stimulus and calcium response in platelets. We also propose a simple model function for calcium peaks, which is in good agreement with the measured data. The proposed technique and model function can be used for in-depth studies of platelet physiology. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Spiryova D.V. , Vorobev A.Y. , Klimontov V.V. , Koroleva E.A. , Moskalensky A.E.
Optical uncaging of ADP reveals the early calcium dynamics in single, freely moving platelets

Biomedical Optics Express. 2020. V.11. N6. P.3319-3329. DOI: 10.1364/BOE.392745 WOS Scopus РИНЦ

Full text from publisher

Published online:	May 27, 2020
Published print:	Jun 1, 2020

Web of science	WOS:000561841000011
Scopus	2-s2.0-85085841096
Elibrary	43294629
OpenAlex	W3025288148