Abstract: Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Cite: Spiryova D.V. , Vorob'ev A.Y. , Moskalensky A.E.
Study of calcium signaling dynamics in single platelets using optical activation methods
Proceedings of SPIE - The International Society for Optical Engineering. 2020. V.11359. 113590U . DOI: 10.1117/12.2559414 WOS Scopus РИНЦ

Dates:

Published print:

Apr 1, 2020

Identifiers:

Web of science	WOS:000674749600021
Scopus	2-s2.0-85090380691
Elibrary	45310946
OpenAlex	W3014860428

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