Cross-linking method using pentaepoxide for improving bovine and porcine bioprosthetic pericardia: A multiparametric assessment study

Cross-linking method using pentaepoxide for improving bovine and porcine bioprosthetic pericardia: A multiparametric assessment study Full article

Journal

Materials Science and Engineering: C
ISSN: 0928-4931 , E-ISSN: 1873-0191

Output data

Year: 2021, Volume: 118, Article number : 111473, Pages count : DOI: 10.1016/j.msec.2020.111473

Tags

Bioprosthetic valve; Bovine pericardium; Cross-linking; Epoxy compounds; Porcine pericardium

Authors

Zhuravleva I.Y. ¹ , Karpova E.V. ² , Oparina L.A. ³ , Poveschenko Olga V. ¹ , Surovtseva Maria A. ¹ , Titov Anatoly T. ⁴ , Ksenofontov A.L. ⁵ , Vasilieva Maria B. ¹ , Kuznetsova E.V. ¹ , Bogachev-Prokophiev A.V. ¹ , Trofimov B.A. ³

Affiliations

1	(Scopus) E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya St., Novosibirsk, 630055, Russian Federation
2	(Scopus) N. Vorozhtsov Institute of Organic Chemistry of SB RAS, 9 Lavrentyev Avenue, Novosibirsk, 630090, Russian Federation
3	(Scopus) A. Favorsky Institute of Chemistry SB RAS, 1 Favorsky St., Irkutsk, 664033, Russian Federation
4	(Scopus) V. Sobolev Institute of Geology and Mineralogy SB RAS, 3 Academician Koptyug Avenue, Novosibirsk, 630090, Russian Federation
5	(Scopus) A. Belozersky Research Institute of Physico-Chemical Biology MSU, House 1, Building 40 Leninskye gory, Moscow, 119992, Russian Federation

Bioprosthetic heart valves made from bovine pericardium (BP) and porcine pericardium (PP) preserved with glutaraldehyde (GA) are commonly used in valve surgeries but prone to calcification in many patients. In this study, we compared BP and PP preserved with GA, ethylene glycol diglycidyl ether (DE), and 1,2,3,4,6-penta-O-{1-[2-(glycidyloxy)ethoxy]ethyl}-D-glucopyranose (PE). We studied the stabilities of DE and PE in preservation media along with the amino acid (AA) compositions, Fourier-transform infrared spectra, mechanical properties, surface morphologies, thermal stability, calcification, and the cytocompatibility of BP and PP treated with 0.625% GA, 5% DE, 2% PE, and alternating 5% DE and 2% PE for 3 + 11 d and 10 + 10 d, respectively. Both epoxides were stable in the water-buffer solutions (pH 7.4). DE provided high linkage densities in BP and PP owing to reactions with Hyl, Lys, His, Arg, Ser, and Tyr. PE reacted weakly with these AAs but strongly with Met. High cross-linking density obtained using the 10 d + 10 d method provided satisfactory thermal stability of biomaterials. The epoxy preservations improved cytocompatibility and resistance to calcification. PE enhanced the stress/strain properties of the xenogeneic pericardia, perhaps by forming nanostructures that were clearly visualised in BP using scanning electron microscopy. The DE + PE combination, in an alternating cross-linking manner, thus constitutes a promising option for developing bioprosthetic pericardia. © 2020 Elsevier B.V.

Zhuravleva I.Y. , Karpova E.V. , Oparina L.A. , Poveschenko O.V. , Surovtseva M.A. , Titov A.T. , Ksenofontov A.L. , Vasilieva M.B. , Kuznetsova E.V. , Bogachev-Prokophiev A.V. , Trofimov B.A.
Cross-linking method using pentaepoxide for improving bovine and porcine bioprosthetic pericardia: A multiparametric assessment study
Materials Science and Engineering: C. 2021. V.118. 111473 . DOI: 10.1016/j.msec.2020.111473 WOS Scopus

Published print:

Jan 1, 2021

Web of science	WOS:000600864900003
Scopus	2-s2.0-85090408946
OpenAlex	W3082957190