Trityl radicals as spin labels | Sciact

Trityl radicals as spin labels Full article

Journal

Electron Paramagnetic Resonance
ISSN: 1464-4622 , E-ISSN: 0305-9758

Output data

Year: 2017, Volume: 25, Pages: 35-60 Pages count : DOI: 10.1039/9781782629436-00035

Authors

Krumkacheva Olesya ^1,2,3 , Bagryanskaya Elena ^1,2

Affiliations

1	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, Pr Lavrentjeva 9, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Pirogova St 2, Novosibirsk 630090, Russia
3	(Данные Web of science) Int Tomog Ctr SB RAS, Inst Skaya 3A, Novosibirsk 630090, Russia

During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.

Krumkacheva O. , Bagryanskaya E.
Trityl radicals as spin labels
Electron Paramagnetic Resonance. 2017. V.25. P.35-60. DOI: 10.1039/9781782629436-00035 WOS Scopus РИНЦ

Web of science	WOS:000414786200003
Scopus	2-s2.0-85006508377
Elibrary	29466832
OpenAlex	W2558067160