Interaction of triarylmethyl radicals with DNA termini revealed by orientation-selective W-band double electron-electron resonance spectroscopy

Interaction of triarylmethyl radicals with DNA termini revealed by orientation-selective W-band double electron-electron resonance spectroscopy Full article

Journal

Physical Chemistry Chemical Physics
ISSN: 1463-9076 , E-ISSN: 1463-9084

Output data

Year: 2016, Volume: 18, Number: 42, Pages: 29549-29554 Pages count : 6 DOI: 10.1039/c6cp05904j

Authors

Fedin Matvey V. ^1,2 , Shevelev Georgiy Yu. ^2,3 , Pyshnyi Dmitrii V. ^2,3 , Tormyshev Victor M. ^2,4 , Jeschke Gunnar ⁵ , Yulikov Maxim ⁵ , Bagryanskaya Elena G. ^2,4

Affiliations

1	(Данные Web of science) Int Tomog Ctr SB RAS, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
3	(Данные Web of science) Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk 630090, Russia
4	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, Novosibirsk 630090, Russia
5	(Данные Web of science) Swiss Fed Inst Technol, Phys Chem Lab, CH-8093 Zurich, Switzerland

Spin labels selectively attached to biomolecules allow high-accuracy nanoscale distance measurements using pulsed electron paramagnetic resonance (EPR), in many cases providing the only access to the structure of complex biosystems. Triarylmethyl (TAM) radicals have recently emerged as a new class of spin labels expanding the applicability of the method to physiological temperatures. Along with other factors, the accuracy of the obtained distances crucially relies on the understanding of interactions between biomolecules and spin labels. In this work, we consider such crucial interactions and their impact on pulsed EPR distance measurements in TAM-labeled DNAs. Using orientation-selective high-frequency (94 GHz) double electron-electron resonance (DEER) we demonstrate strong specific interactions between DNA termini and TAM labels, leading to a significant restriction of their conformational mobility. An understanding of such interactions guides the way to select optimum TAM-labeling strategies, thus refining nanoscale EPR distance measurements in nucleic acids and their complexes under physiological conditions.

Fedin M.V. , Shevelev G.Y. , Pyshnyi D.V. , Tormyshev V.M. , Jeschke G. , Yulikov M. , Bagryanskaya E.G.
Interaction of triarylmethyl radicals with DNA termini revealed by orientation-selective W-band double electron-electron resonance spectroscopy

Physical Chemistry Chemical Physics. 2016. V.18. N42. P.29549-29554. DOI: 10.1039/c6cp05904j WOS Scopus РИНЦ

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