Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR

Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR Full article

Journal

Angewandte Chemie - International Edition
ISSN: 1433-7851 , E-ISSN: 1521-3773

Output data

Year: 2016, Volume: 55, Number: 38, Pages: 11538-11542 Pages count : 5 DOI: 10.1002/anie.201606335

Tags

EPR; membrane proteins; PELDOR or DEER; spin labeling; trityl

Authors

Joseph Benesh ^1,2 , Tormyshev Victor M. ^3,4 , Rogozhnikova Olga Yu. ³ , Akhmetzyanov Dmitry ^1,2 , Bagryanskaya Elena G. ³ , Prisner Thomas F. ^1,2

Affiliations

1	(Данные Web of science) Goethe Univ Frankfurt, Inst Phys & Theoret Chem, Max von Laue Str 7, D-60438 Frankfurt, Germany
2	(Данные Web of science) Goethe Univ Frankfurt, Biomol Magnetresonanz Zentrum, Max von Laue Str 7, D-60438 Frankfurt, Germany
3	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, Novosibirsk 630090, Russia
4	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia

The orchestrated interaction of transmembrane proteins with other molecules mediates several crucial biological processes. Detergent solubilization may significantly alter or even abolish such hetero-oligomeric interactions, which makes observing them at high resolution in their native environment technically challenging. Dipolar electron paramagnetic resonance (EPR) techniques such as pulsed electro-electron double resonance (PELDOR) can provide very precise distances within biomolecules. To concurrently determine the inter-subunit interaction and the intra-subunit conformational changes in hetero-oligomeric complexes, a combination of different spin labels is required. Orthogonal spin labeling using a triarylmethyl (TAM) label in combination with a nitroxide label is used to detect protein-ligand interactions in native lipid bilayers. This approach provides a higher sensitivity and total selectivity and will greatly facilitate the investigation of multimeric transmembrane complexes employing different spin labels in the native lipid environment.

Joseph B. , Tormyshev V.M. , Rogozhnikova O.Y. , Akhmetzyanov D. , Bagryanskaya E.G. , Prisner T.F.
Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
Angewandte Chemie - International Edition. 2016. V.55. N38. P.11538-11542. DOI: 10.1002/anie.201606335 WOS Scopus РИНЦ

Published online:	Aug 11, 2016
Published print:	Sep 12, 2016

Web of science	WOS:000383748900040
Scopus	2-s2.0-84994223670
Elibrary	27573910
OpenAlex	W2515575745