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New diagnostic capabilities for crohns disease: use of fatty acid profiles of erythrocyte membranes and blood serum Full article

Journal United European Gastroenterology Journal
ISSN: 2050-6406 , E-ISSN: 2050-6414
Output data Year: 2020, Volume: 8, Number: S8, Pages: 334-335 Pages count : 2
Tags CROHN'S DISEASE, FATTY ACIDS, DIAGNOSIS
Authors Kruchinina M.V. 1 , Gromov A.A. 1 , Svetlova I.O. 1 , Azgaldyan A.V. 1 , Kruchinin V.N. 2 , Shashkov M.V. 3 , Sokolova A.S. 4
Affiliations
1 Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 10, Lavrentjev avenue, Novosibirsk, 630090 RUSSIA
2 Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences, 13, Acad. Lavrentjeva avenue, Novosibirsk, 630090
3 Boreskov Institute of Catalysis, Siberian Branch of the Russian Academy of Sciences, 5, Lavrent'ev prospect, Novosibirsk, 630090
4 Vorozhtzov Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Science, 9, Academician Lavrentiev Ave., Novosibirsk, 630090

Abstract: The fatty acids, in particular the n-3 and n-6 PUFAs, mediate a number of key biological processes, including production of eicosanoids, inflammation, physiological processes in the cell membrane, gene regulation and expression [1]. Aims & Methods: The purpose of this research is to investigate the features of the fatty acid composition of erythrocyte membranes (Er) and blood serum (BS) among patients with Crohn´s Disease (CD) for possible use in differential diagnosis. We have examined 22 patients with CD, 21 patients with inflammatory bowel diseases unclassified (IBDU) (35.2+4.7 years), and 20 healthy individuals, which are comparable in age and gender to the main groups. The study of the composition of fatty acids (FAs) of Er and BS has been carried out using a gas chromatography-mass spectrometry (GC/MS) system based on the Agilent 7000B Triple Quadrupole (USA). Results: The levels of most saturated, monounsaturated omega-9 (palmitoleic C16:1n-9, oleinic C18:1n-9), omega-6 PUFAs have been significantly higher among patients with Crohn´s disease, and the predominant part of omega-3 (including eicosapentaenoic (C20:5 n-3), docosapentaenoic (C22:5 n-3), docosahexaenoic (C22:6 n-3) is lower than among healthy individuals (p< 0.001-0.05). The relative content of FAs among patients with inflammatory bowel disease unclassified has happened to be interim in comparison with those for patients with CD and healthy ones. The revealed features of the FA levels of Er and BS membranes, as well as their ratios among patients with CD, are promising for the purposes of differential diagnosis, including in cases of indeterminate colitis.
Cite: Kruchinina M.V. , Gromov A.A. , Svetlova I.O. , Azgaldyan A.V. , Kruchinin V.N. , Shashkov M.V. , Sokolova A.S.
New diagnostic capabilities for crohns disease: use of fatty acid profiles of erythrocyte membranes and blood serum
United European Gastroenterology Journal. 2020. V.8. NS8. P.334-335. РИНЦ
Identifiers:
Elibrary 44090669