Global LC-MS/MS targeted metabolomics using a combination of HILIC and RP LC separation modes on an organic monolithic column based on 1-vinyl-1,2,4-triazole

Global LC-MS/MS targeted metabolomics using a combination of HILIC and RP LC separation modes on an organic monolithic column based on 1-vinyl-1,2,4-triazole Научная публикация

Журнал

Talanta
ISSN: 0039-9140

Вых. Данные

Год: 2024, Том: 267, Номер: 15 January 2024, Номер статьи : 125168, Страниц : 12 DOI: 10.1016/j.talanta.2023.125168

Ключевые слова

Liquid chromatography; Tandem mass spectrometry; Metabolomics; Dual retention chromatography; Monolithic column; X-ray irradiation

Авторы

Basov Nikita V. ^1,2 , Rogachev Artem D. ^1,2 , Aleshkova Maria A. ^1,2 , Gaisler Evgeny V. ^1,2 , Sotnikova Yulia S. ^1,2,3 , Patrushev Yuri V. ^2,3 , Tolstikova Tatiana G. ^1,2 , Yarovaya Olga I. ^1,2 , Pokrovsky Andrey G. ² , Salakhutdinov Nariman F. ^1,2

Организации

1	N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, 630090, Novosibirsk, Russia
2	Novosibirsk State University, Pirogov Str., 2, 630090, Novosibirsk, Russia
3	Boreskov Institute of Catalysis, Acad. Lavrentiev Ave., 5, 630090, Novosibirsk, Russia

Информация о финансировании (1)

Российский Научный Фонд

22-23-01068

The paper presents an LC-MS/MS-based approach to targeted screening of both polar and non-polar metabolites using a synthesized monolithic column which is a copolymer of styrene, divinylbenzene, and 1-vinyl-1,2,4-triazole. It was shown that this column in combination with eluents 20 mM (NH4)2CO3 + NH3 (pH = 9.8, eluent A) and ACN (eluent B) allows for separation of metabolites of different nature in two modes, HILIC and RP LC, and these methods are mutually complementary. A combination of analyses based on these two modes was proposed, allowing detection of about 400 metabolites in a total time of less than 30 min. Comparison of the developed method with those utilizing commercially available columns with sorbents of various types showed that it could provide a broader metabolite coverage. Using the developed approach, metabolomic screening of dried blood spots samples of mice exposed with X-ray was performed, and metabolites that could be considered as possible markers of irradiation exposure and organ tissue damage were detected. Analysis of marker metabolites revealed metabolic pathways that were altered by radiation exposure. Comparison of the results with literature data showed the effectiveness of the developed metabolomic screening approach.

Basov N.V. , Rogachev A.D. , Aleshkova M.A. , Gaisler E.V. , Sotnikova Y.S. , Patrushev Y.V. , Tolstikova T.G. , Yarovaya O.I. , Pokrovsky A.G. , Salakhutdinov N.F.
Global LC-MS/MS targeted metabolomics using a combination of HILIC and RP LC separation modes on an organic monolithic column based on 1-vinyl-1,2,4-triazole
Talanta. 2024. V.267. N15 January 2024. 125168 :1-12. DOI: 10.1016/j.talanta.2023.125168 WOS Scopus РИНЦ OpenAlex

Поступила в редакцию:	1 июн. 2023 г.
Принята к публикации:	4 сент. 2023 г.
Опубликована online:	6 сент. 2023 г.
Опубликована в печати:	15 янв. 2024 г.

Web of science:	WOS:001080329800001
Scopus:	2-s2.0-85171749087
РИНЦ:	60053354
OpenAlex:	W4386483746

БД	Цитирований
OpenAlex	10
Web of science	6
Scopus	3
РИНЦ	11