Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus Full article
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Pharmaceuticals
ISSN: 1424-8247 |
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| Output data | Year: 2022, Volume: 15, Number: 9, Article number : 1158, Pages count : 11 DOI: 10.3390/ph15091158 | ||||
| Tags | antiviral activity; coumarin; terpene; LC-MS/MS; pharmacokinetics; RSV | ||||
| Authors |
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| Affiliations |
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Funding (1)
| 1 | Российский Научный Фонд | 21-13-00026 |
Abstract:
The stability of a new coumarin derivative, agent K-142, bearing -pinene residue and
possessing antiviral activity against respiratory syncytial virus (RSV) was studied in whole mice blood
in vitro, and a method for its quantification in this matrix was developed and validated. The sample
preparation method was precipitation of whole blood with a mixture of 0.2 M ZnSO4 with MeOH
(2:8 v/v) containing 2-adamantylamine hydrochloride as an internal standard (IS). Analysis was
carried out by HPLC-MS/MS using reversed phase chromatography and a triple quadrupole mass
spectrometer 6500 QTRAP (SCIEX) in multiple reaction monitoring (MRM) mode. The transitions
351.2 ! 217.1 Da and 152.2 ! 93.1/107.2 Da were monitored for K-142 and the IS, respectively.
The method was validated in terms of selectivity, calibration curve, LLOQ, accuracy and precision,
stability, recovery and carry over. The developed method was used for a pharmacokinetics study of
the compound after its oral administration to mice at a dose of 20 mg/kg.
Cite:
Nemolochnova A.G.
, Rogachev A.D.
, Сальникова О.П.
, Khomenko T.M.
, Volcho K.
, Yarovaya O.I.
, Fatianova A.V.
, Pokrovsky A.G.
, Salakhutdinov N.F.
Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus
Pharmaceuticals. 2022. V.15. N9. 1158 :1-11. DOI: 10.3390/ph15091158 WOS Scopus РИНЦ OpenAlex
Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus
Pharmaceuticals. 2022. V.15. N9. 1158 :1-11. DOI: 10.3390/ph15091158 WOS Scopus РИНЦ OpenAlex
Dates:
| Submitted: | Jul 31, 2022 |
| Published print: | Sep 14, 2022 |
| Published online: | Sep 18, 2022 |
Identifiers:
| Web of science: | WOS:000856673400001 |
| Scopus: | 2-s2.0-85138667180 |
| Elibrary: | 54068029 |
| OpenAlex: | W4296350909 |