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WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley Научная публикация

Журнал International Journal of Molecular Sciences
ISSN: 1661-6596
Вых. Данные Год: 2023, Том: 24, Номер: 7, Страницы: 6762 Страниц : 1 DOI: 10.3390/ijms24076762
Ключевые слова CRISPR-associated endonculeases; cuticle; gene cluster; lipids; targeted mutagenesis; transcription factor; wax
Авторы Gerasimova Sophia V. 1 , Kolosovskaya Ekaterina V. 2 , Vikhorev Alexander V. 2 , Korotkova Anna M. 1 , Hertig Christian W. 3 , Genaev Mikhail A. 1 , Domrachev Dmitry V. 4 , Morozov Sergey V. 4 , Chernyak Elena I. 4 , Shmakov Nikolay A. 1 , Vasiliev Gennady V. 1 , Kochetov Alex V. 1 , Kumlehn Jochen 3 , Khlestkina Elena K. 2
Организации
1 Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
2 Vavilov Institute of General Genetics
3 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Germany, Gatersleben
4 N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences

Реферат: Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the Arabidopsis thaliana AtWIN1 gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the HvWIN1 gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for win1 knockout (KO) and cer-x mutants showed that Cer-X and WIN1 are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of win1 KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known Cer-cqu locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the Cer-cqu gene cluster.
Библиографическая ссылка: Gerasimova S.V. , Kolosovskaya E.V. , Vikhorev A.V. , Korotkova A.M. , Hertig C.W. , Genaev M.A. , Domrachev D.V. , Morozov S.V. , Chernyak E.I. , Shmakov N.A. , Vasiliev G.V. , Kochetov A.V. , Kumlehn J. , Khlestkina E.K.
WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley
International Journal of Molecular Sciences. 2023. V.24. N7. P.6762. DOI: 10.3390/ijms24076762 WOS РИНЦ OpenAlex
Даты:
Поступила в редакцию: 21 февр. 2023 г.
Принята к публикации: 27 мар. 2023 г.
Опубликована в печати: 5 апр. 2023 г.
Идентификаторы БД:
Web of science: WOS:000970162800001
РИНЦ: 50753113
OpenAlex: W4362619848
Цитирование в БД:
БД Цитирований
OpenAlex 5
Web of science 5
РИНЦ 7
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