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Microimaging of Oxygen Concentration near Live Photosynthetic Cells by Electron Spin Resonance Научная публикация

Журнал Biophysical Journal
ISSN: 0006-3495
Вых. Данные Год: 2010, Том: 99, Номер: 3, Страницы: 971-978 Страниц : DOI: 10.1016/j.bpj.2010.05.002
Авторы Halevy Revital 1 , Tormyshev Victor 2,3 , Blank Aharon 1
Организации
1 (Данные Web of science) Technion Israel Inst Technol, Schulich Fac Chem, Haifa, Israel
2 (Данные Web of science) Novosibirsk Organ Chem Inst, Novosibirsk, Russia
3 (Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia

Реферат: We present what is, to our knowledge, a new methodology for high-resolution three-dimensional imaging of oxygen concentration near live cells. The cells are placed in the buffer solution of a stable paramagnetic probe, and electron spin-resonance microimaging is employed to map out the probe's spin-spin relaxation time (T-2). This information is directly linked to the concentration of the oxygen molecule. The method is demonstrated with a test sample and with a small amount of live photosynthetic cells (cyanobacteria), under conditions of darkness and light. Spatial resolution of similar to 30 x 30 x 100 Am is demonstrated, with similar to mu M oxygen concentration sensitivity and sub-fmol absolute oxygen sensitivity per voxel. The use of electron spin-resonance microimaging for oxygen mapping near cells complements the currently available techniques based on micro-electrodes or fluorescence/phosphorescence. Furthermore, with the proper paramagnetic probe, it will also be readily applicable for intracellular oxygen microimaging, a capability which other methods find very difficult to achieve.
Библиографическая ссылка: Halevy R. , Tormyshev V. , Blank A.
Microimaging of Oxygen Concentration near Live Photosynthetic Cells by Electron Spin Resonance
Biophysical Journal. 2010. V.99. N3. P.971-978. DOI: 10.1016/j.bpj.2010.05.002 WOS Scopus РИНЦ OpenAlex
Файлы: Полный текст от издателя
Даты:
Опубликована в печати: 1 авг. 2010 г.
Идентификаторы БД:
Web of science: WOS:000280693100034
Scopus: 2-s2.0-77955706570
РИНЦ: 15336807
OpenAlex: W2101270641
Цитирование в БД:
БД Цитирований
Web of science 23
Scopus 24
РИНЦ 26
OpenAlex 26
Альметрики: