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Superoxide-mediated reduction of the nitroxide group can prevent detection of nitric oxide by nitronyl nitroxides Научная публикация

Журнал Free Radical Research
ISSN: 1071-5762
Вых. Данные Год: 1997, Том: 26, Номер: 1, Страницы: 7-17 Страниц : 11 DOI: 10.3109/10715769709097780
Ключевые слова nitric oxide; superoxide; free radical; nitronyl nitroxide; hydroxylamine; electron spin resonance; chemiluminescence; endothelial cells; microsomes; liposomes
Авторы Haseloff Reiner F. 1 , Zollner Stefan 2 , Kirilyuk Igor A. 2 , Grigorev Igor A. 2 , Reszka Regina 3 , Bernhardt Rita 3 , Mertsch Katharina 1 , Roloff Birgit 1 , Blasig Ingolf E. 1
Организации
1 Forschungsinstitut für Molekulare Pharmakologie, Delbrück-Zentrum für Molekulare Medizin, Robert-Rössle-Str.10, 13125 Berlin, Germany
2 Max-Delbrück-Zentrum für Molekulare Medizin, Robert-Rössle-Str.10, 13125 Berlin, Germany
3 N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences

Реферат: Nitronyl nitroxides (NN), a class of compounds which react with nitric oxide forming imino nitroxides, were applied in different systems for the detection of nitric oxide. Addition of a NN to planar monolayers of bovine aortic endothelial cells (BAEC) activated by Ca2+ ionophore A23187 immediately resulted in a strong decrease of the ozone-mediated (NO)-N-. chemiluminescence. Simultaneously, a rapid diminution of the electron spin resonance (ESR) signal intensity of the NN (without detectable formation of the corresponding imino nitroxide) was observed; superoxide dismutase partially inhibited this decrease in the NN concentration. Model experiments using hypoxanthine/xanthine oxidase in aqueous solution and KO2 in dimethylsulfoxide as sources of O-2(.-) revealed that there is a rapid reduction of nitronyl nitroxides by superoxide. The second order rate constant for the reaction of the water soluble NN with O2(.-) was determined to be 8.8 . 10(5) M(-1)s(-1), which is more than two orders of magnitude higher than the value reported previously for reaction with (NO)-N-. (Woldman et al., BBRC 202, 195-203, 1994). Reduction of the nitronyl nitroxide was also observed in the presence of glutathione, ascorbic acid or rabbit liver microsomes. Incorporation of both nitronyl and imino nitroxides into liposomes strongly decreased reduction by superoxide and other reductants, however, in the presence of microsomes, there was no protective effect by liposomal encapsulation of NN. The results indicate that in biological systems (in addition to other reducing agents) the presence of superoxide can prevent the detection of nitric oxide using nitronyl nitroxides.
Библиографическая ссылка: Haseloff R.F. , Zollner S. , Kirilyuk I.A. , Grigorev I.A. , Reszka R. , Bernhardt R. , Mertsch K. , Roloff B. , Blasig I.E.
Superoxide-mediated reduction of the nitroxide group can prevent detection of nitric oxide by nitronyl nitroxides
Free Radical Research. 1997. V.26. N1. P.7-17. DOI: 10.3109/10715769709097780 WOS Scopus РИНЦ OpenAlex
Даты:
Опубликована в печати: 1 янв. 1997 г.
Опубликована online: 7 июл. 2009 г.
Идентификаторы БД:
Web of science: WOS:A1997WB99800002
Scopus: 2-s2.0-0031029067
РИНЦ: 13259031
OpenAlex: W2135051362
Цитирование в БД:
БД Цитирований
Web of science 33
Scopus 36
OpenAlex 35
Альметрики: