Abstract: Spin trapping compounds are used frequently to detect free radicals released by cells. Their cytotoxicity has to be considered in order to prevent perturbations of normal cell growth and viability. Eleven spin traps (eight nitrones and three nitroso traps) have been tested for their effects on bovine aortic endothelial cells (toxicity range, 50% survival rate), The lowest cytotoxicity was found for 5,5-dimethylpyrroline-1-oxide and 2,2,4-trimethyl-2H-imidazole-1-oxide whereas nitrosobenzene and 2-methyl-2-nitrosopropane exerted the strongest cytotoxic effects. In addition, three nitronyl nitroxides mere tested, Their cytotoxicity was found to be dependent on substitution, and the toxic concentration of a lipophilic derivative was found to be more than two orders lower as compared to a hydrophilic derivative. The results of this study indicate that most spin traps can be used in cell cultures at customary (i.e. millimolar) concentrations; caution is recommended when nitroso spin traps are applied to cells. (C) 1997 Federation of European Biochemical Societies.

Cite: Haseloff R.F. , Mertsch K. , Rohde E. , Baeger I. , Grigorev I.A. , Blasig I.E.
Cytotoxicity of spin trapping compounds
FEBS Letters. 1997. V.418. N1-2. P.73-75. DOI: 10.1016/S0014-5793(97)01349-5 WOS Scopus РИНЦ OpenAlex

Dates:

Published print:	Nov 24, 1997
Published online:	Dec 3, 1997

Identifiers:

Web of science:	WOS:A1997YJ81800017
Scopus:	2-s2.0-0030842255
Elibrary:	13254016
OpenAlex:	W2081603712

Citing:

DB	Citing
Web of science	50
Scopus	53
Elibrary	53
OpenAlex	50

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