Abstract: Nitric oxide (NO) has been shown to be an important mediator in vasodilation, neurotransmission and cellular cytotoxicity. We investigated a new series of nitronylnitroxyl radicals (NNR) as spin traps for NO. It was found these radicals react with NO with rate constants of about 10(4) M(-1)c(-1) forming stable iminonitroxides with dramatic changes in EPR spectra. To overcome fast reduction of the radicals (a few seconds in rat cerebella cytosol), NNR with charged trimethylammoniophenyl group (Ib) was incorporated into the inner volume of large unilamellar phosphatidylcholine liposomes. In this case the reduction of the radical Ib in rat cerebella cytosol is slow (ca. 1% per min). The rate of NO production by NO synthase from rat cerebellum measured by NNR, Ib, is in a reasonable agreement with that obtained by spectrophotometric method. (C) 1994 Academic Press, Inc.

Cite: Woldman Y.Y. , Khramtsov V.V. , Grigorev I.A. , Kiriljuk I.A. , Utepbergenov D.I.
SPIN-TRAPPING OF NITRIC-OXIDE BY NITRONYLNITROXIDES - MEASUREMENT OF THE ACTIVITY OF NO SYNTHASE FROM RAT CEREBELLUM
Biochemical and Biophysical Research Communications. 1994. V.202. N1. P.195-203. DOI: 10.1006/bbrc.1994.1912 WOS Scopus РИНЦ OpenAlex

Dates:

Published print:

Jul 1, 1994

Identifiers:

Web of science:	WOS:A1994NX14200028
Scopus:	2-s2.0-0028022842
Elibrary:	31081933
OpenAlex:	W2059989483

Citing:

DB	Citing
Web of science	61
Scopus	67
Elibrary	68
OpenAlex	65

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