Oxidative damage to epigenetically methylated sites affects DNA stability, dynamics and enzymatic demethylation

Oxidative damage to epigenetically methylated sites affects DNA stability, dynamics and enzymatic demethylation Научная публикация

Журнал

Nucleic Acids Research
ISSN: 0305-1048 , E-ISSN: 1362-4962

Вых. Данные

Год: 2018, Том: 46, Номер: 20, Страницы: 10827-10839 Страниц : 13 DOI: 10.1093/nar/gky893

Авторы

Gruber David R. ¹ , Toner Joanna J. ¹ , Miears Heather L. ¹ , Shernyukov Andrey V. ^2,3 , Kiryutin Alexey S. ^3,4 , Lomzov Alexander A. ^3,5 , Endutkin Anton V. ^3,5 , Grin Inga R. ^3,5 , Petrova Darya V. ^3,5 , Kupryushkin Maxim S. ⁵ , Yurkovskaya Alexandra V. ^3,4 , Johnson Eric C. ⁶ , Okon Mark ^7,8 , Bagryanskaya Elena G. ^2,3 , Zharkov Dmitry O. ^3,5 , Smirnov Serge L. ¹

Организации

1	(Данные Web of science) Western Washington Univ, Chem Dept, 516 High St, Bellingham, WA 98225 USA
2	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem SB RAS, 9 Lavrentieva Ave, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, 2 Pirogova St, Novosibirsk 630090, Russia
4	(Данные Web of science) SB RAS Int Tomog Ctr, 3a Inst Skaya St, Novosibirsk 630090, Russia
5	(Данные Web of science) SB RAS Inst Chem Biol & Fundamental Med, 8 Lavrentieva Ave, Novosibirsk 630090, Russia
6	(Данные Web of science) Bruker Biospin, 61 Daggett Dr, San Jose, CA 95134 USA
7	(Данные Web of science) Univ British Columbia, Dept Biochem & Mol Biol, Dept Chem, Vancouver, BC V6T 1Z3, Canada
8	(Данные Web of science) Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z3, Canada

DNA damage can affect various regulatory elements of the genome, with the consequences for DNA structure, dynamics, and interaction with proteins remaining largely unexplored. We used solution NMR spectroscopy, restrained and free molecular dynamics to obtain the structures and investigate dominant motions for a set of DNA duplexes containing CpG sites permuted with combinations of 5-methylcytosine (mC), the primary epigenetic base, and 8-oxoguanine (oxoG), an abundant DNA lesion. Guanine oxidation significantly changed the motion in both hemimethylated and fully methylated DNA, increased base pair breathing, induced BI -> BII transition in the backbone 3' to the oxoG and reduced the variability of shift and tilt helical parameters. UV melting experiments corroborated the NMR and molecular dynamics results, showing significant destabilization of all methylated contexts by oxoG. Notably, some dynamic and thermodynamic effects were not additive in the fully methylated oxidized CpG, indicating that the introduced modifications interact with each other. Finally, we show that the presence of oxoG biases the recognition of methylated CpG dinucleotides by ROS1, a plant enzyme involved in epigenetic DNA demethylation, in favor of the oxidized DNA strand. Thus, the conformational and dynamic effects of spurious DNA oxidation in the regulatory CpG dinucleotide can have far-reaching biological consequences.

Gruber D.R. , Toner J.J. , Miears H.L. , Shernyukov A.V. , Kiryutin A.S. , Lomzov A.A. , Endutkin A.V. , Grin I.R. , Petrova D.V. , Kupryushkin M.S. , Yurkovskaya A.V. , Johnson E.C. , Okon M. , Bagryanskaya E.G. , Zharkov D.O. , Smirnov S.L.
Oxidative damage to epigenetically methylated sites affects DNA stability, dynamics and enzymatic demethylation

Nucleic Acids Research. 2018. V.46. N20. P.10827-10839. DOI: 10.1093/nar/gky893 WOS Scopus РИНЦ OpenAlex

Полный текст от издателя

Опубликована online:

5 окт. 2018 г.

Web of science:	WOS:000456709700028
Scopus:	2-s2.0-85056609090
РИНЦ:	41783801
OpenAlex:	W2895652499

БД	Цитирований
Web of science	27
Scopus	27
РИНЦ	26
OpenAlex	31