Реферат: OBJECTIVES: The development of calcification-resistant bioprosthetic materials is a very important challenge for paediatric surgery. The subcutaneous implantation in rats is the well-known first-stage model for this kind of research. Using this model, we aimed to compare calcification of the porcine aortic wall and bovine pericardium and jugular vein wall cross-linked with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (DE). We also determined the efficacy of DE-preserved tissue modification with 2-(2-carboxyethylamino)ethylidene-1,1-bisphosphonic acid (CEABA). METHODS: Three groups of each biomaterial were evaluated: GA-treated, DE-treated and DE+ CEABA-treated. The microstructure of non-implanted biomaterials was assessed by light microscopy after Picro Mallory staining; the phosphorus content of the DE and DE+ CEABA samples was assessed by atomic emission spectrometry. Samples were implanted subcutaneously into young rats for 10 and 60 days. The explant end-point included quantitative calcification assessment by atomic absorption spectrophotometry and light microscopy examination after von Kossa staining. RESULTS: All GA-treated biomaterials had a high calcium-binding capacity (>100 mu g/mg dry tissue). DE preservation decreased the vein wall and pericardium calcium content by 4- and 40-fold, respectively, but was ineffective for the aortic wall. The calculated CEABA content was almost equal in the vein wall and pericardium (17.7 and 18.5 mu M/g) and slightly less in the aortic wall (15 mu M/g) (P= 0.011). CEABA effectively reduced mineralization in the DE aortic wall and DE pericardium to 10.1 (7.8-21.1) and 0.95 (0.57-1.38) mu g/mg but had no effect in the DE vein wall. Mineralization in the GA- and DE-treated aortic and vein walls was predominantly associated with elastin. CEABA modification decreased elastin calcification but did not block it completely. CONCLUSIONS: Each xenogeneic material requires individual anticalcification strategy. DE + CEABA pretreatment demonstrates a high mineralization-blocking efficacy for the bovine pericardium and should be employed to further develop the paediatric pericardial conduit. Aortic wall calcification cannot be blocked completely using this strategy.

Библиографическая ссылка: Zhuravleva I.Y. , Nichay N.R. , Kulyabin Y.Y. , Timchenko T.P. , Korobeinikov A.A. , Polienko Y.F. , Shatskaya S.S. , Kuznetsova E.V. , Voitov A.V. , Bogachev-Prokophiev A.V. , Karaskov A.M.
In search of the best xenogeneic material for a paediatric conduit: an experimental study
Interactive Cardiovascular and Thoracic Surgery. 2018. V.26. N5. P.738-744. DOI: 10.1093/icvts/ivx445 WOS Scopus OpenAlex

Файлы: Полный текст от издателя

Даты:

Опубликована online:	15 янв. 2018 г.
Опубликована в печати:	1 мая 2018 г.

Идентификаторы БД:

Web of science:	WOS:000432288000004
Scopus:	2-s2.0-85047178368
OpenAlex:	W2784041422

Цитирование в БД:

БД	Цитирований
Web of science	12
Scopus	16
OpenAlex	16

Альметрики: