Room-temperature distance measurements using RIDME and the orthogonal spin labels trityl/nitroxide

Room-temperature distance measurements using RIDME and the orthogonal spin labels trityl/nitroxide Full article

Journal

Physical Chemistry Chemical Physics
ISSN: 1463-9076 , E-ISSN: 1463-9084

Output data

Year: 2018, Volume: 20, Number: 15, Pages: 10224-10230 Pages count : 7 DOI: 10.1039/c8cp01093e

Authors

Kuzhelev Andrey A. ^1,2,3 , Krumkacheva Olesya A. ^2,3 , Shevelev Georgiy Yu. ^3,4 , Yulikov Maxim ⁵ , Fedin Matvey V. ^2,3 , Bagryanskaya Elena G. ^1,3

Affiliations

1	(Данные Web of science) RAS, NN Vorozhtsov Novosibirsk Inst Organ Chem SB, Novosibirsk 630090, Russia
2	(Данные Web of science) RAS, Int Tomog Ctr SB, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
4	(Данные Web of science) RAS, Inst Chem Biol & Fundamental Med SB, Novosibirsk 630090, Russia
5	(Данные Web of science) Swiss Fed Inst Technol, Lab Phys Chem, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland

Electron paramagnetic resonance (EPR) based nanometer distance measurements at ambient temperatures are of particular interest for structural biology applications. The nitroxide spin labels commonly used in EPR reveal relatively short transverse relaxation under these conditions, which limits their use for detecting static dipolar interactions. At the same time, the longitudinal relaxation of nitroxide spin labels is still long enough to allow using them as pumped' species in the relaxation induced dipolar modulation enhancement (RIDME) experiment where the detection is carried out on the slower relaxing triarylmethyl (TAM) spin labels. In the present study, we report the first demonstration of room-temperature RIDME distance measurements in nucleic acids using TAM as the slow-relaxing detected species and traditional nitroxide as the fast-relaxing partner spin. Two types of immobilizers, glassy trehalose and the modified silica gel Nucleosil, were used for immobilization of the spin-labeled biomolecules. The room-temperature RIDME-based distance distributions are in good agreement with those measured at 80 K by other techniques. Room-temperature RIDME on the spin pairs trityl/nitroxide may become a useful method for the structural characterization of biomacromolecules and biomolecular complexes at near physiological temperatures.

Kuzhelev A.A. , Krumkacheva O.A. , Shevelev G.Y. , Yulikov M. , Fedin M.V. , Bagryanskaya E.G.
Room-temperature distance measurements using RIDME and the orthogonal spin labels trityl/nitroxide
Physical Chemistry Chemical Physics. 2018. V.20. N15. P.10224-10230. DOI: 10.1039/c8cp01093e WOS Scopus РИНЦ OpenAlex

Web of science:	WOS:000430537600055
Scopus:	2-s2.0-85045851046
Elibrary:	35541603
OpenAlex:	W2789232302

DB	Citing
Web of science	33
Scopus	34
Elibrary	34
OpenAlex	35