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Electron Paramagnetic Resonance Measurements of Reactive Oxygen Species by Cyclic Hydroxylamine Spin Probes Full article

Journal Antioxidants and Redox Signaling
ISSN: 1523-0864 , E-ISSN: 1557-7716
Output data Year: 2018, Volume: 28, Number: 15, Pages: 1433-1443 Pages count : 11 DOI: 10.1089/ars.2017.7396
Tags electron paramagnetic resonance; reactive oxygen species; superoxide; hydroxylamine spin probes; electron spin resonance
Authors Dikalov Sergey I. 1 , Polienko Yuliya F. 2,3 , Kirilyuk Igor 2,3
Affiliations
1 (Данные Web of science) Vanderbilt Univ, Med Ctr, Dept Med, Div Clin Pharmacol, Nashville, TN USA
2 (Данные Web of science) Novosibirsk Organ Chem Inst, Lab Nitrogen Cpds, Novosibirsk, Russia
3 (Данные Web of science) Novosibirsk State Univ, Dept Organ Chem, Novosibirsk, Russia

Abstract: Significance: Oxidative stress contributes to numerous pathophysiological conditions such as development of cancer, neurodegenerative, and cardiovascular diseases. A variety of measurements of oxidative stress markers in biological systems have been developed; however, many of these methods are not specific, can produce artifacts, and do not directly detect the free radicals and reactive oxygen species (ROS) that cause oxidative stress. Electron paramagnetic resonance (EPR) is a unique tool that allows direct measurements of free radical species. Cyclic hydroxylamines are useful and convenient molecular probes that readily react with ROS to produce stable nitroxide radicals, which can be quantitatively measured by EPR. In this work, we critically review recent applications of various cyclic hydroxylamine spin probes in biology to study oxidative stress, their advantages, and the shortcomings. Recent Advances: In the past decade, a number of new cyclic hydroxylamine spin probes have been developed and their successful application for ROS measurement using EPR has been published. These new state-of-the-art methods provide improved selectivity and sensitivity for in vitro and in vivo studies. Critical Issues: Although cyclic hydroxylamine spin probes EPR application has been previously described, there has been lack of translation of these new methods into biomedical research, limiting their widespread use. This work summarizes "best practice" in applications of cyclic hydroxylamine spin probes to assist with EPR studies of oxidative stress. Future Directions: Additional studies to advance hydroxylamine spin probes from the "basic science" to biomedical applications are needed and could lead to better understanding of pathological conditions associated with oxidative stress.
Cite: Dikalov S.I. , Polienko Y.F. , Kirilyuk I.
Electron Paramagnetic Resonance Measurements of Reactive Oxygen Species by Cyclic Hydroxylamine Spin Probes
Antioxidants and Redox Signaling. 2018. V.28. N15. P.1433-1443. DOI: 10.1089/ars.2017.7396 WOS Scopus РИНЦ OpenAlex
Files: Full text from publisher
Dates:
Published print: May 20, 2018
Identifiers:
Web of science: WOS:000417108900001
Scopus: 2-s2.0-85045506809
Elibrary: 35519440
OpenAlex: W2769163423
Citing:
DB Citing
Web of science 81
Scopus 85
OpenAlex 102
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