Analysis of the enzymatic activity of individual subcellular particles of Acetabularia mediterranea

Analysis of the enzymatic activity of individual subcellular particles of Acetabularia mediterranea Full article

Journal

Analytical Biochemistry
ISSN: 0003-2697

Output data

Year: 1976, Volume: 71, Number: 1, Pages: 1-5 Pages count : DOI: 10.1016/0003-2697(76)90002-6

Authors

Mikichur N. I. , Sandakhchiev L. S. , Shchelkunov S. N.

Affiliations

1	(Scopus) Institute of Organic Chemistry, Siberian Division of the Academy of Sciences of the U.S.S.R., Novosibirsk, 90, U.S.S.R.

A simple procedure is described for quantitative spectrophotometric analysis of individual subcellular particles; the procedure is based upon the method proposed earlier to measure the optical density of microdroplets in capillary flow cells. The activity of malate dehydrogenase (MDH) was determined in individual chloroplasts, nuclei, nucleoli, and nuclear membranes of the unicellularalga Acetabularia. Chloroplasts are markedly heterogeneous in their enzymatic activity. A high MDH activity is characteristic of nuclei, insignificant activity is found in nuclear membranes, and no activity is present in nucleoli. © 1976.

Mikichur N.I. , Sandakhchiev L.S. , Shchelkunov S.N.
Analysis of the enzymatic activity of individual subcellular particles of Acetabularia mediterranea
Analytical Biochemistry. 1976. V.71. N1. P.1-5. DOI: 10.1016/0003-2697(76)90002-6 WOS Scopus OpenAlex

Published print:

Mar 1, 1976

Web of science:	WOS:A1976BP05500001
Scopus:	2-s2.0-0017169188
OpenAlex:	W1985990476

DB	Citing
Scopus	1
Web of science	3
OpenAlex	3