Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model

Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model Научная публикация

Журнал

Scientific Reports
ISSN: 2045-2322

Вых. Данные

Год: 2017, Том: 7, Номер статьи : 46686, Страниц : DOI: 10.1038/srep46686

Авторы

Khodanovich Marina Yu ¹ , Sorokina Irina V. ² , Glazacheva Valentina Yu ¹ , Akulov Andrey E. ^1,3 , Nemirovich-Danchenko Nikolay M. ¹ , Romashchenko Alexander V. ³ , Tolstikova Tatyana G. ² , Mustafina Lilia R. ⁴ , Yarnykh Vasily L. ^1,5

Организации

1	(Данные Web of science) Tomsk State Univ, Res Inst Biol & Biophys, Tomsk, Russia
2	(Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Organ Chem, Novosibirsk, Russia
3	(Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk, Russia
4	(Данные Web of science) Siberian State Med Univ, Tomsk, Russia
5	(Данные Web of science) Univ Washington, Dept Radiol, Seattle, WA 98195 USA

Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained in vivo on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control.57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p < 0.05) demyelination in cuprizone-treated animals was found according to both LFB staining and MPF in all anatomical structures (corpus callosum, anterior commissure, internal capsule, thalamus, caudoputamen, and cortex). MPF strongly correlated with quantitative histology in all animals (r = 0.95, p < 0.001) as well as in treatment and control groups taken separately (r = 0.96, p = 0.002 and r = 0.93, p = 0.007, respectively). Close agreement between histological myelin staining and MPF suggests that fast MPF mapping enables robust and accurate quantitative assessment of demyelination in both WM and GM.

Khodanovich M.Y. , Sorokina I.V. , Glazacheva V.Y. , Akulov A.E. , Nemirovich-Danchenko N.M. , Romashchenko A.V. , Tolstikova T.G. , Mustafina L.R. , Yarnykh V.L.
Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model

Scientific Reports. 2017. V.7. 46686 . DOI: 10.1038/srep46686 WOS Scopus OpenAlex

Полный текст от издателя

Опубликована online:	24 апр. 2017 г.
Опубликована в печати:	1 мая 2017 г.

Web of science:	WOS:000400057800001
Scopus:	2-s2.0-85029596727
OpenAlex:	W2608116788

БД	Цитирований
Web of science	69
Scopus	69
OpenAlex	85